ArticlePloS one2010
Stable morphology, but dynamic internal reorganisation, of interphase human chromosomes in living cells.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 64 citations in OpenAlex.
- Differential regulation of mesoscale chromosome conformations in osteoblasts and osteosarcoma.Genome biology · 2025Article
- Non-random spatial organization of telomeres varies during the cell cycle and requires LAP2 and BAF.iScience · 2024Article
- Nuclear organisation and replication timing are coupled through RIF1-PP1 interaction.Nature communications · 2021Article
- Poly(ADP-ribose)-dependent chromatin unfolding facilitates the association of DNA-binding proteins with DNA at sites of damage.Nucleic acids research · 2019Article
- Chromosome territories and the global regulation of the genome.Genes, chromosomes & cancer · 2019Review
- Large-scale 3D chromatin reconstruction from chromosomal contacts.BMC genomics · 2019Article
- The structural and functional roles of CTCF in the regulation of cell type-specific and human disease-associated super-enhancers.Genes & genomics · 2019Review
- How epigenome drives chromatin folding and dynamics, insights from efficient coarse-grained models of chromosomes.PLoS computational biology · 2018Article
- 3C and 3C-based techniques: the powerful tools for spatial genome organization deciphering.Molecular cytogenetics · 2018Review
- Transcription-dependent radial distribution of TCF7L2 regulated genes in chromosome territories.Chromosoma · 2017Article
- Single-Cell and Single-Molecule Analysis of Gene Expression Regulation.Annual review of genetics · 2016Review
- The arrangement of Brachypodium distachyon chromosomes in interphase nuclei.Journal of experimental botany · 2016Article
- Resetting a functional G1 nucleus after mitosis.Chromosoma · 2016Review
- Large-scale probabilistic 3D organization of human chromosome territories.Human molecular genetics · 2016Article
- Chromosomes at Work: Organization of Chromosome Territories in the Interphase Nucleus.Journal of cellular biochemistry · 2016Article
- An Overview of Genome Organization and How We Got There: from FISH to Hi-C.Microbiology and molecular biology reviews : MMBR · 2015Review
- Regulation of recombination and genomic maintenance.Cold Spring Harbor perspectives in biology · 2015Review
- Chromatin at the nuclear periphery and the regulation of genome functions.Histochemistry and cell biology · 2015Review
- Spatial genome organization: contrasting views from chromosome conformation capture and fluorescence in situ hybridization.Genes & development · 2014Article
- Gene density and chromosome territory shape.Chromosoma · 2014Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Despite the distinctive structure of mitotic chromosomes, it has not been possible to visualise individual chromosomes in living interphase cells, where chromosomes spend over 90% of their time. Studies of interphase chromosome structure and dynamics use fluorescence in-situ hybridisation (FISH) on fixed cells, which potentially damages structure and loses dynamic information. We have developed a new methodology, involving photoactivation of labelled histone H3 at mitosis, to visualise individual and specific human chromosomes in living interphase cells. Our data revealed bulk chromosome volume and morphology are established rapidly after mitosis, changing only incrementally after the first hour of G1. This contrasted with the behaviour of specific loci on labelled chromosomes, which showed more progressive reorganisation, and revealed that "looping out" of chromatin from chromosome territories is a dynamic state. We measured considerable heterogeneity in chromosome decondensation, even between sister chromatids, which may reflect local structural impediments to decondensation and could potentially amplify transcriptional noise. Chromosome structure showed tremendous resistance to inhibitors of transcription, histone deacetylation and chromatin remodelling. Together, these data indicate steric constraints determine structure, rather than innate chromosome architecture or function-driven anchoring, with interphase chromatin organisation governed primarily by opposition between needs for decondensation and the space available for this to happen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.