ArticleJournal of visualized experiments : JoVE2010
DiOLISTIC labeling of neurons from rodent and non-human primate brain slices.
Article in Journal of visualized experiments : JoVE, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
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- Building and Characterization of an Affordable diOlistic Device for Single-Cell Labeling in Rodent and Non-Human Primate Brain Slices.Current protocols · 2023Article
- Impaired synaptic transmission in dorsal dentate gyrus increases impulsive alcohol seeking.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023Article
- Neurodevelopmental Processes in the Prefrontal Cortex Derailed by Chronic HIV-1 Viral Protein Exposure.Cells · 2021Article
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- RRY Inhibits Amyloid-βJournal of Alzheimer's disease reports · 2021Article
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- Seasonal changes in NRF2 antioxidant pathway regulates winter depression-like behavior.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture.Journal of visualized experiments : JoVE · 2020Article
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- Reduced Hippocampal Dendrite Branching, Spine Density and Neurocognitive Function in Premature Rabbits, and Reversal with Estrogen or TrkB Agonist Treatment.Cerebral cortex (New York, N.Y. : 1991) · 2019Article
- Opioid and chemokine regulation of cortical synaptodendritic damage in HIV-associated neurocognitive disorders.Brain research · 2019Review
- Disruption of Timing: NeuroHIV Progression in the Post-cART Era.Scientific reports · 2019Article
- A Model of ΔBiological psychiatry · 2018Article
- Unraveling Individual Differences In The HIV-1 Transgenic Rat: Therapeutic Efficacy Of Methylphenidate.Scientific reports · 2018Article
- Diolistic Labeling and Analysis of Dendritic Spines.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Synaptic Connectivity in Medium Spiny Neurons of the Nucleus Accumbens: A Sex-Dependent Mechanism Underlying Apathy in the HIV-1 Transgenic Rat.Frontiers in behavioral neuroscience · 2018Article
- Restoring striatal WAVE-1 improves maze exploration performance of GluN1 knockdown mice.PloS one · 2018Article
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Authors and funding
5 authors.
Funding
Abstract
DiOLISTIC staining uses the gene gun to introduce fluorescent dyes, such as DiI, into neurons of brain slices (Gan et al., 2009; O'Brien and Lummis, 2007; Gan et al., 2000). Here we provide a detailed description of each step required together with exemplary images of good and bad outcomes that will help when setting up the technique. In our experience, a few steps proved critical for the successful application of DiOLISTICS. These considerations include the quality of the DiI-coated bullets, the extent of fixative exposure, and the concentration of detergent used in the incubation solutions. Tips and solutions for common problems are provided. This is a versatile labeling technique that can be applied to multiple animal species at a wide range of ages. Unlike other fluorescent labeling techniques that are limited to preparations from young animals or restricted to mice because they rely on the expression of a fluorescent transgene, DiOLISTIC labeling can be applied to animals of all ages, species and genotypes and it can be used in combination with immunostaining to identify a specific subpopulation of cells. Here we demonstrate the use of DiOLISTICS to label neurons in brain slices from adult mice and adult non-human primates with the purpose of quantifying dendrite branching and dendritic spine morphology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.