ArticleProceedings of the National Academy of Sciences of the United States of America2010
Length control of the injectisome needle requires only one molecule of Yop secretion protein P (YscP).
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Pseudomonas syringae Type III Secretion Protein HrpP Manipulates Plant Immunity To Promote Infection.Microbiology spectrum · 2023Article
- Functional analysis of ascP in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence.Journal of microbiology (Seoul, Korea) · 2022Article
- Comparative transcriptomic analysis provides insights into transcription mechanisms of Vibrio parahaemolyticus T3SS during interaction with HeLa cells.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- The PopN Gate-keeper Complex Acts on the ATPase PscN to Regulate the T3SS Secretion Switch from Early to Middle Substrates in Pseudomonas aeruginosa.Journal of molecular biology · 2020Article
- A Structure-Function-Inhibition Analysis of theJournal of bacteriology · 2020Article
- T3S injectisome needle complex structures in four distinct states reveal the basis of membrane coupling and assembly.Nature microbiology · 2019Article
- Structural and Functional Characterization of the Type Three Secretion System (T3SS) Needle ofFrontiers in microbiology · 2019Article
- Hook length of the bacterial flagellum is optimized for maximal stability of the flagellar bundle.PLoS biology · 2018Article
- Assembly, structure, function and regulation of type III secretion systems.Nature reviews. Microbiology · 2017Review
- The Structure of a Type 3 Secretion System (T3SS) Ruler Protein Suggests a Molecular Mechanism for Needle Length Sensing.The Journal of biological chemistry · 2016Article
- Type Three Secretion System in Attaching and Effacing Pathogens.Frontiers in cellular and infection microbiology · 2016Review
- Intracellular Scaling Mechanisms.Cold Spring Harbor perspectives in biology · 2015Review
- Molecular ruler determines needle length for the Salmonella Spi-1 injectisome.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A coiled-coil domain acts as a molecular ruler to regulate O-antigen chain length in lipopolysaccharide.Nature structural & molecular biology · 2015Article
- Article
- Role of EscP (Orf16) in injectisome biogenesis and regulation of type III protein secretion in enteropathogenic Escherichia coli.Journal of bacteriology · 2012Article
- Rebuttal: flagellar hook length is controlled by a secreted molecular ruler.Journal of bacteriology · 2012Article
- Flagellar hook length is controlled by a secreted molecular ruler.Journal of bacteriology · 2012Article
- Needle length control and the secretion substrate specificity switch are only loosely coupled in the type III secretion apparatus of Shigella.Microbiology (Reading, England) · 2012Article
- Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.Microbiology and molecular biology reviews : MMBR · 2012Review
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The needle length of the Yersinia spp. injectisome is determined by Yop secretion protein P (YscP), an early substrate of the injectisome itself. There is a linear correlation between the length of YscP and the length of the needle, suggesting that YscP acts as a molecular ruler. However, it is not known whether one single molecule of YscP suffices to control the length of one needle or whether several molecules of YscP are exported in alternation with the needle subunit YscF until the needle length matches the ruler length, which would stop needle growth. To address this question, three different strains expressing simultaneously a short and a long version of YscP were engineered. The experimentally obtained needle length distribution was compared with the distributions predicted by stochastic modeling of the various possible scenarios. The experimental data are compatible with the single ruler model and not with the scenarios involving more than one ruler per needle.
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