ArticleProceedings of the National Academy of Sciences of the United States of America2010
Single-cell mechanics provides a sensitive and quantitative means for probing amyloid-beta peptide and neuronal cell interactions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 80 citations in OpenAlex.
- A computational pipeline for spatial mechano-transcriptomics.Nature methods · 2025Article
- FLIM-Phasor Analysis (FLIM-ϕ) of Aβ-Induced Membrane Order Alterations: Towards a Cell-Based Biosensor for Early Alzheimer's Disease Diagnosis.Micromachines · 2025Article
- Atomic Force Microscopy for the Study of Cell Mechanics in Pharmaceutics.Pharmaceutics · 2024Review
- Ligand Binding-Induced Cellular Membrane Deformation is Correlated with the Changes in Membrane Stiffness.The journal of physical chemistry. B · 2023Article
- Insights in Cell Biomechanics through Atomic Force Microscopy.Materials (Basel, Switzerland) · 2023Review
- The Curcumin Derivative GT863 Protects Cell Membranes in Cytotoxicity by Aβ Oligomers.International journal of molecular sciences · 2023Article
- Laser-Structured Si and PLGA Inhibit the Neuro2a Differentiation in Mono- and Co-Culture with Glia.Tissue engineering and regenerative medicine · 2023Article
- Combating amyloid-induced cellular toxicity and stiffness by designer peptidomimetics.RSC chemical biology · 2022Article
- Beta-amyloid pore linked to controlled calcium influx into the cell: A new paradigm for Alzheimer's Disease.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2022Article
- Article
- Direct Observations of Silver Nanowire-Induced Frustrated Phagocytosis among NR8383 Lung Alveolar Macrophages.The journal of physical chemistry. B · 2020Article
- High Mannose N-Glycans Promote Migration of Bone-Marrow-Derived Mesenchymal Stromal Cells.International journal of molecular sciences · 2020Article
- A Soft Mechanical Phenotype of SH-SY5Y Neuroblastoma and Primary Human Neurons Is Resilient to Oligomeric Aβ(1-42) Injury.ACS chemical neuroscience · 2020Article
- Endogenously generated amyloid-β increases stiffness in human neuroblastoma cells.European biophysics journal : EBJ · 2017Article
- Brillouin flow cytometry for label-free mechanical phenotyping of the nucleus.Lab on a chip · 2017Article
- AFM study shows prominent physical changes in elasticity and pericellular layer in human acute leukemic cells due to inadequate cell-cell communication.Nanotechnology · 2016Article
- Amyloid beta oligomers induce neuronal elasticity changes in age-dependent manner: a force spectroscopy study on living hippocampal neurons.Scientific reports · 2016Article
- Membrane roughness as a sensitive parameter reflecting the status of neuronal cells in response to chemical and nanoparticle treatments.Journal of nanobiotechnology · 2016Article
- Rheological properties of cells measured by optical tweezers.BMC biophysics · 2016Article
- Kinetics of small molecule interactions with membrane proteins in single cells measured with mechanical amplification.Science advances · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
By using a highly sensitive technique of atomic force microscopy-based single-cell compression, the rigidity of cultured N2a and HT22 neuronal cells was measured as a function of amyloid-beta42 (Abeta42) protein treatment. Abeta42 oligomers led to significant cellular stiffening; for example, 90-360% higher force was required to reach 80% deformation for N2a cells. Disaggregated or fibrillar forms of Abeta42 showed much less change. These observations were explained by a combination of two factors: (i) incorporation of oligomer into cellular membrane, which resulted in an increase in the Young's modulus of the membrane from 0.9+/-0.4 to 1.85+/-0.75 MPa for N2a cells and from 1.73+/-0.90 to 5.5+/-1.4 MPa for HT22 cells, and (ii) an increase in intracellular osmotic pressure (e.g., from 7 to 40 Pa for N2a cells) through unregulated ion influx. These findings and measurements provide a deeper, more characteristic, and quantitative insight into interactions between cells and Abeta42 oligomers, which have been considered the prime suspect for initiating neuronal dysfunction in Alzheimer's disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.