ArticleProceedings of the National Academy of Sciences of the United States of America2010
Microhomology-mediated and nonhomologous repair of a double-strand break in the chloroplast genome of Arabidopsis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 56 citations in OpenAlex.
- Article
- Editing and synthetic engineering of chloroplast genomes.Plant communications · 2026Review
- Complex plastome structural variation caused by intermolecular and intramolecular recombination accounts for leaf variegation inPlant diversity · 2026Article
- Identification of a highly efficient chloroplast-targeting peptide for plastid engineering.PLoS biology · 2024Article
- Leaf variegation caused by plastome structural variation: an example fromHorticulture research · 2024Article
- Engineering the plastid and mitochondrial genomes of flowering plants.Nature plants · 2022Review
- Plastid Phylogenomic Analysis of Tordylieae Tribe (Apiaceae, Apioideae).Plants (Basel, Switzerland) · 2022Article
- Functional peptide-mediated plastid transformation in tobacco, rice, and kenaf.Frontiers in plant science · 2022Article
- Plastome Structural Evolution and Homoplastic Inversions in Neo-Astragalus (Fabaceae).Genome biology and evolution · 2021Article
- Extensive genomic rearrangements mediated by repetitive sequences in plastomes of Medicago and its relatives.BMC plant biology · 2021Article
- RNase H1C collaborates with ssDNA binding proteins WHY1/3 and recombinase RecA1 to fulfill the DNA damage repair in Arabidopsis chloroplasts.Nucleic acids research · 2021Article
- Advancing organelle genome transformation and editing for crop improvement.Plant communications · 2021Review
- An Insight Into the Mechanism of Plant Organelle Genome Maintenance and Implications of Organelle Genome in Crop Improvement: An Update.Frontiers in cell and developmental biology · 2021Review
- Plastid Genomes of Flowering Plants: Essential Principles.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The Dark Side of UV-Induced DNA Lesion Repair.Genes · 2020Review
- Review
- Microhomologies Are Associated with Tandem Duplications and Structural Variation in Plant Mitochondrial Genomes.Genome biology and evolution · 2020Article
- DNA Repair and the Stability of the Plant Mitochondrial Genome.International journal of molecular sciences · 2020Review
- Article
- Plant organellar DNA polymerases repair double-stranded breaks by microhomology-mediated end-joining.Nucleic acids research · 2019Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplast DNA (cpDNA) is under great photooxidative stress, yet its evolution is very conservative compared with nuclear or mitochondrial genomes. It can be expected that DNA repair mechanisms play important roles in cpDNA survival and evolution, but they are poorly understood. To gain insight into how the most severe form of DNA damage, a double-strand break (DSB), is repaired, we have developed an inducible system in Arabidopsis that employs a psbA intron endonuclease from Chlamydomonas, I-CreII, that is targeted to the chloroplast using the rbcS1 transit peptide. In Chlamydomonas, an I-CreII-induced DSB in psbA was repaired, in the absence of the intron, by homologous recombination between repeated sequences (20-60 bp) abundant in that genome; Arabidopsis cpDNA is very repeat poor, however. Phenotypically strong and weak transgenic lines were examined and shown to correlate with I-CreII expression levels. Southern blot hybridizations indicated a substantial loss of DNA at the psbA locus, but not cpDNA as a whole, in the strongly expressing line. PCR analysis identified deletions nested around the I-CreII cleavage site indicative of DSB repair using microhomology (6-12 bp perfect repeats, or 10-16 bp with mismatches) and no homology. These results provide evidence of alternative DSB repair pathways in the Arabidopsis chloroplast that resemble the nuclear, microhomology-mediated and nonhomologous end joining pathways, in terms of the homology requirement. Moreover, when taken together with the results from Chlamydomonas, the data suggest an evolutionary relationship may exist between the repeat structure of the genome and the organelle's ability to repair broken chromosomes.
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