ArticleJournal of biotechnology2010
Binding characterization of determinants in porcine aminopeptidase N, the cellular receptor for transmissible gastroenteritis virus.
Article in Journal of biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Identification of GRP78 as a novel host factor that facilitates zoonotic porcine deltacoronavirus internalization and replication via clathrin-mediated endocytosis.Journal of virology · 2026Article
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- Review
- Different Infectivity of Swine Enteric Coronaviruses in Cells of Various Species.Pathogens (Basel, Switzerland) · 2024Article
- Inhibition of porcine deltacoronavirus entry and replication by Cepharanthine.Virus research · 2024Article
- Improvements in pig agriculture through gene editing.CABI agriculture and bioscience · 2022Review
- Porcine enteric coronaviruses: an updated overview of the pathogenesis, prevalence, and diagnosis.Veterinary research communications · 2021Review
- Resistance to coronavirus infection in amino peptidase N-deficient pigs.Transgenic research · 2019Article
- Transferrin receptor 1 is a supplementary receptor that assists transmissible gastroenteritis virus entry into porcine intestinal epithelium.Cell communication and signaling : CCS · 2018Article
- Establishment of a nanoparticle-assisted RT-PCR assay to distinguish field strains and attenuated strains of porcine epidemic diarrhea virus.Archives of virology · 2016Article
- Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus.Virus research · 2015Article
- Screening and antiviral analysis of phages that display peptides with an affinity to subunit C of porcine aminopeptidase.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2013Article
- Development of porcine rotavirus vp6 protein based ELISA for differentiation of this virus and other viruses.Virology journal · 2013Article
- Generation of a mouse scFv library specific for porcine aminopeptidase N using the T7 phage display system.Journal of virological methods · 2012Article
- Virus-binding activity of the truncated C subunit of porcine aminopeptidase N expressed in Escherichia coli.Biotechnology letters · 2012Article
- Article
- Reverse transcription loop-mediated isothermal amplification for rapid detection of transmissible gastroenteritis virus.Current microbiology · 2011Article
- Phage displayed peptides recognizing porcine aminopeptidase N inhibit transmissible gastroenteritis coronavirus infection in vitro.Virology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Four truncated porcine aminopeptidase N (pAPN, a cellular receptor for porcine coronaviruses) proteins were expressed in prokaryotic cells. The recognizing of a specific serum against pAPN to these proteins was investigated by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. The binding ability of the proteins to transmissible gastroenteritis virus (TGEV), a porcine coronavirus, was analyzed by ELISA. The inhibitory effect of these proteins to cell infection by TGEV was analyzed using plaque assays. Our data indicate that three truncated pAPNs positively reacted with the specific antiserum and the major binding regions of pAPN were limited in regions 36aa-223aa, 349aa-591aa and 592-963aa. The proteins showed discrepant binding activity to either pAPN antibody or TGE virions. Moreover, the truncated proteins blocked the infection of cells by TGEV to different extent. The results suggest that the major antibody-binding domains of pAPN may associate with the receptor-binding determinants. The role of APN is discussed in the context of virus receptor usage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.