ArticleBiophysical journal2010
Biophysical characterization of an ensemble of intramolecular i-motifs formed by the human c-MYC NHE III1 P1 promoter mutant sequence.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 43 citations in OpenAlex.
- The interactions of Pu22 G-quadruplex, derived fromMolecular therapy. Nucleic acids · 2025Article
- In vivo visualization of the i-motif DNA secondary structure in the Bombyx mori testis.Epigenetics & chromatin · 2020Article
- Study of conformational transitions of i-motif DNA using time-resolved fluorescence and multivariate analysis methods.Nucleic acids research · 2019Article
- BmILF and i-motif structure are involved in transcriptional regulation of BmPOUM2 in Bombyx mori.Nucleic acids research · 2018Article
- The Consequences of Overlapping G-Quadruplexes and i-Motifs in the Platelet-Derived Growth Factor Receptor β Core Promoter Nuclease Hypersensitive Element Can Explain the Unexpected Effects of Mutations and Provide Opportunities for Selective Targeting of Both Structures by Small Molecules To Downregulate Gene Expression.Journal of the American Chemical Society · 2017Article
- Probing the Potential Role of Non-B DNA Structures at Yeast Meiosis-Specific DNA Double-Strand Breaks.Biophysical journal · 2017Article
- The structure of an endogenous Drosophila centromere reveals the prevalence of tandemly repeated sequences able to form i-motifs.Scientific reports · 2015Article
- Regulation of tyrosine hydroxylase transcription by hnRNP K and DNA secondary structure.Nature communications · 2014Article
- The dynamic character of the BCL2 promoter i-motif provides a mechanism for modulation of gene expression by compounds that bind selectively to the alternative DNA hairpin structure.Journal of the American Chemical Society · 2014Article
- The effect of molecular crowding on the stability of human c-MYC promoter sequence I-motif at neutral pH.Molecules (Basel, Switzerland) · 2013Article
- Bcl-2 promoter sequence G-quadruplex interactions with three planar and non-planar cationic porphyrins: TMPyP4, TMPyP3, and TMPyP2.PloS one · 2013Article
- DSC deconvolution of the structural complexity of c-MYC P1 promoter G-quadruplexes.Biophysical journal · 2011Article
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
i-Motif-forming sequences are present in or near the regulatory regions of >40% of all genes, including known oncogenes. We report here the results of a biophysical characterization and computational study of an ensemble of intramolecular i-motifs that model the polypyrimidine sequence in the human c-MYC P1 promoter. Circular dichroism results demonstrate that the mutant sequence (5'-CTT TCC TAC CCTCCC TAC CCT AA-3') can adopt multiple "i-motif-like," classical i-motif, and single-stranded structures as a function of pH. The classical i-motif structures are predominant in the pH range 4.2-5.2. The "i-motif-like" and single-stranded structures are the most significant species in solution at pH higher and lower, respectively, than that range. Differential scanning calorimetry results demonstrate an equilibrium mixture of at least three i-motif folded conformations with Tm values of 38.1, 46.6, and 49.5 degrees C at pH 5.0. The proposed ensemble of three folded conformations includes the three lowest-energy conformations obtained by computational modeling and two folded conformers that were proposed in a previous NMR study. The NMR study did not report the most stable conformer found in this study.
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