ArticleBiophysical journal2010
Cholesterol-dependent nanomechanical stability of phase-segregated multicomponent lipid bilayers.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Cis glycan-glycan interactions organize membrane nanodomains that tune receptor signaling.Nature communications · 2026Article
- Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques?Membranes · 2025Review
- Unveiling the mechanism of essential oil action against skin pathogens: from ancient wisdom to modern science.Archives of microbiology · 2024Review
- Mechanics of biomimetic free-standing lipid membranes: insights into the elasticity of complex lipid compositions.RSC advances · 2024Article
- Temperature-Induced Restructuring of Mycolic Acid Bilayers Modeling theMolecules (Basel, Switzerland) · 2024Article
- Structural diversity of photoswitchable sphingolipids for optodynamic control of lipid microdomains.Biophysical journal · 2023Article
- Characterization of Phase Separated Planar Lipid Bilayer Membrane by Fluorescence Ratio Imaging and Scanning Probe Microscope.Membranes · 2022Article
- Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients.International journal of molecular sciences · 2022Article
- Formation and Nanoscale Characterization of Asymmetric Supported Lipid Bilayers Containing Raft-Like Domains.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Atomic force spectroscopy with magainin 1 functionalized tips and biomimetic supported lipid membranes.European biophysics journal : EBJ · 2022Article
- Lipid Self-Assemblies under the Atomic Force Microscope.International journal of molecular sciences · 2021Review
- Altered Membrane Mechanics Provides a Receptor-Independent Pathway for Serotonin Action.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- 25-Hydroxycholesterol Effect on Membrane Structure and Mechanical Properties.International journal of molecular sciences · 2021Article
- Chemically synthesized GbEuropean biophysics journal : EBJ · 2021Review
- Interleaflet Decoupling in a Lipid Bilayer at Excess Cholesterol Probed by Spectroscopic Ellipsometry and Simulations.The Journal of membrane biology · 2020Article
- Lamellar Phases Composed of Phospholipid, Cholesterol, and Ceramide, as Studied byBiophysical journal · 2019Article
- Molecular AFM imaging of Hsp70-1A association with dipalmitoyl phosphatidylserine reveals membrane blebbing in the presence of cholesterol.Cell stress & chaperones · 2018Article
- Modulation of lipid membrane structural and mechanical properties by a peptidomimetic derived from reduced amide scaffold.Biochimica et biophysica acta. Biomembranes · 2017Article
- Review
- Effects of Low Concentrations of Docosahexaenoic Acid on the Structure and Phase Behavior of Model Lipid Membranes.Membranes · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cholesterol is involved in endocytosis, exocytosis, and the assembly of sphingolipid/cholesterol-enriched domains, as has been demonstrated in both model membranes and living cells. In this work, we explored the influence of different cholesterol levels (5-40 mol%) on the morphology and nanomechanical stability of phase-segregated lipid bilayers consisting of dioleoylphosphatidylcholine/sphingomyelin/cholesterol (DOPC/SM/Chol) by means of atomic force microscopy (AFM) imaging and force mapping. Breakthrough forces were consistently higher in the SM/Chol-enriched liquid-ordered domains (Lo) than in the DOPC-enriched fluid-disordered phase (Ld) at a series of loading rates. We also report the activation energies (DeltaEa) for the formation of an AFM-tip-induced fracture, calculated by a model for the rupture of molecular thin films. The obtained DeltaEa values agree remarkably well with reported values for fusion-related processes using other techniques. Furthermore, we observed that within the Chol range studied, the lateral organization of bilayers can be categorized into three distinct groups. The results are rationalized by fracture nanomechanics of a ternary phospholipid/sphingolipid/cholesterol mixture using correlated AFM-based imaging and force mapping, which demonstrates the influence of a wide range of cholesterol content on the morphology and nanomechanical stability of model bilayers. This provides fundamental insights into the role of cholesterol in the formation and stability of sphingolipid/cholesterol-enriched domains, as well as in membrane fusion.
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