ArticleBiophysical journal2010
Is the in vitro ejection of bacteriophage DNA quasistatic? A bulk to single virus study.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Temperature-dependent ejection evolution arising from active and passive effects in DNA viruses.Biophysical journal · 2024Article
- Article
- Role of DNA-DNA sliding friction and nonequilibrium dynamics in viral genome ejection and packaging.Nucleic acids research · 2023Article
- Structural basis of bacteriophage T5 infection trigger andScience advances · 2023Article
- Biological foundations of successful bacteriophage therapy.EMBO molecular medicine · 2022Review
- Article
- Time-resolved DNA release from an O-antigen-specificThe Journal of biological chemistry · 2019Article
- Article
- Exploring the Balance between DNA Pressure and Capsid Stability in Herpesviruses and Phages.Journal of virology · 2015Article
- Ionic switch controls the DNA state in phage λ.Nucleic acids research · 2015Article
- Conformational changes leading to T7 DNA delivery upon interaction with the bacterial receptor.The Journal of biological chemistry · 2015Article
- Solid-to-fluid-like DNA transition in viruses facilitates infection.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Length quantization of DNA partially expelled from heads of a bacteriophage T3 mutant.Virology · 2014Article
- OmpA and OmpC are critical host factors for bacteriophage Sf6 entry in Shigella.Molecular microbiology · 2014Article
- Topological friction strongly affects viral DNA ejection.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Popping the cork: mechanisms of phage genome ejection.Nature reviews. Microbiology · 2013Review
- Langevin dynamics simulation of DNA ejection from a phage.Journal of biological physics · 2013Article
- Polymorphism of DNA conformation inside the bacteriophage capsid.Journal of biological physics · 2013Article
- Article
- A single-molecule Hershey-Chase experiment.Current biology : CB · 2012Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteriophage T5 DNA ejection is a complex process that occurs on several timescales in vitro. By using a combination of bulk and single phage measurements, we quantitatively study the three steps of the ejection-binding to the host receptor, channel-opening, and DNA release. Each step is separately addressed and its kinetics parameters evaluated. We reconstruct the bulk kinetics from the distribution of single phage events by following individual DNA molecules with unprecedented time resolution. We show that, at the single phage level, the ejection kinetics of the DNA happens by rapid transient bursts that are not correlated to any genome sequence defects. We speculate that these transient pauses are due to local phase transitions of the DNA inside the capsid. We predict that such pauses should be seen for other phages with similar DNA packing ratios.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.