ArticleBiophysical journal2010
Superresolution localization of single functional IP3R channels utilizing Ca2+ flux as a readout.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Stochastic model of IP3-induced Ca2+ spiking of HEK293 cells.PLoS computational biology · 2025Article
- Dissociation of inositol 1,4,5-trisphosphate from IPThe Journal of biological chemistry · 2023Article
- CaCell calcium · 2022Article
- Termination of CaCell calcium · 2021Article
- KRAP tethers IPNature communications · 2021Article
- Noise analysis of cytosolic calcium image data.Cell calcium · 2020Article
- Spatial-temporal patterning of CaSeminars in cell & developmental biology · 2019Review
- Simulation of calcium signaling in fine astrocytic processes: Effect of spatial properties on spontaneous activity.PLoS computational biology · 2019Article
- All three IPScience signaling · 2018Article
- Dynamic CaCell calcium · 2018Article
- Comparison of CaCell calcium · 2017Article
- Termination of calcium puffs and coupled closings of inositol trisphosphate receptor channels.Cell calcium · 2014Article
- Single-molecule tracking of inositol trisphosphate receptors reveals different motilities and distributions.Biophysical journal · 2014Article
- Frequency and relative prevalence of calcium blips and puffs in a model of small IP₃R clusters.Biophysical journal · 2014Article
- Fluorophore localization algorithms for super-resolution microscopy.Nature methods · 2014Review
- Article
- Towards the Physics of Calcium Signalling in Plants.Plants (Basel, Switzerland) · 2013Review
- Inverse problems in neuronal calcium signaling.Journal of mathematical biology · 2013Article
- The probability of triggering calcium puffs is linearly related to the number of inositol trisphosphate receptors in a cluster.Biophysical journal · 2012Article
- Differential distribution, clustering, and lateral diffusion of subtypes of the inositol 1,4,5-trisphosphate receptor.The Journal of biological chemistry · 2011Article
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Authors and funding
3 authors.
Funding
Abstract
The subcellular localization of membrane Ca2+ channels is crucial for their functioning, but is difficult to study because channels may be distributed more closely than the resolution of conventional microscopy is able to detect. We describe a technique, stochastic channel Ca2+ nanoscale resolution (SCCaNR), employing Ca2+-sensitive fluorescent dyes to localize stochastic openings and closings of single Ca2+-permeable channels within <50 nm, and apply it to examine the clustered arrangement of inositol trisphosphate receptor (IP3R) channels underlying local Ca2+ puffs. Fluorescence signals (blips) arising from single functional IP3Rs are almost immotile (diffusion coefficient<0.003 microm2 s(-1)), as are puff sites over prolonged periods, suggesting that the architecture of this signaling system is stable and not subject to rapid, dynamic rearrangement. However, rapid stepwise changes in centroid position of fluorescence are evident within the durations of individual puffs. These apparent movements likely result from asynchronous gating of IP3Rs distributed within clusters that have an overall diameter of approximately 400 nm, indicating that the nanoscale architecture of IP3R clusters is important in shaping local Ca2+ signals. We anticipate that SCCaNR will complement superresolution techniques such as PALM and STORM for studies of Ca2+ channels as it obviates the need for photoswitchable labels and provides functional as well as spatial information.
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