ArticleBMC genomics2010
Array comparative genomic hybridization analysis of Trichoderma reesei strains with enhanced cellulase production properties.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 103 citations in OpenAlex.
- Robust Production of Cellulases to Develop a Low-Cost Sugar Platform.Advances in biochemical engineering/biotechnology · 2026Review
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- Regulatory Role of Vacuolar Calcium Transport Proteins in Growth, Calcium Signaling, and Cellulase Production inJournal of fungi (Basel, Switzerland) · 2024Article
- Transgressive phenotypes from outbreeding between theMicrobiology spectrum · 2024Article
- Enhanced heterologous gene expression in Trichoderma reesei by promoting multicopy integration.Applied microbiology and biotechnology · 2024Article
- The protein methyltransferase TrSAM inhibits cellulase gene expression by interacting with the negative regulator ACE1 in Trichoderma reesei.Communications biology · 2024Article
- Effect of the res2 transcription factor gene deletion on protein secretion and stress response in the hyperproducer strain Trichoderma reesei Rut-C30.BMC microbiology · 2023Article
- Genetic Transformation of Trichoderma spp.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.BMC genomics · 2020Article
- CRZ1 regulator and calcium cooperatively modulate holocellulases gene expression in Trichoderma reesei QM6a.Genetics and molecular biology · 2020Article
- Enhancement of cellulase production in Trichoderma reesei RUT-C30 by comparative genomic screening.Microbial cell factories · 2019Article
- Strain improvement ofFood science and biotechnology · 2019Article
- Protein hyperproduction in fungi by design.Applied microbiology and biotechnology · 2018Review
- Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fungi.Applied microbiology and biotechnology · 2018Review
- Article
- Proximity ligation scaffolding and comparison of twoBiotechnology for biofuels · 2017Article
- Abundance of Secreted Proteins ofFrontiers in microbiology · 2017Article
- Genome sequencing and transcriptome analysis ofBiotechnology for biofuels · 2017Article
- The Relation Between Promoter Chromatin Status, Xyr1 and Cellulase Ex-pression in Trichoderma reesei.Current genomics · 2016Article
- Fungal-mediated consolidated bioprocessing: the potential of Fusarium oxysporum for the lignocellulosic ethanol industry.AMB Express · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTrichoderma reesei is the main industrial producer of cellulases and hemicellulases that are used to depolymerize biomass in a variety of biotechnical applications. Many of the production strains currently in use have been generated by classical mutagenesis. In this study we characterized genomic alterations in high-producing mutants of T. reesei by high-resolution array comparative genomic hybridization (aCGH). Our aim was to obtain genome-wide information which could be utilized for better understanding of the mechanisms underlying efficient cellulase production, and would enable targeted genetic engineering for improved production of proteins in general.
resultsWe carried out an aCGH analysis of four high-producing strains (QM9123, QM9414, NG14 and Rut-C30) using the natural isolate QM6a as a reference. In QM9123 and QM9414 we detected a total of 44 previously undocumented mutation sites including deletions, chromosomal translocation breakpoints and single nucleotide mutations. In NG14 and Rut-C30 we detected 126 mutations of which 17 were new mutations not documented previously. Among these new mutations are the first chromosomal translocation breakpoints identified in NG14 and Rut-C30. We studied the effects of two deletions identified in Rut-C30 (a deletion of 85 kb in the scaffold 15 and a deletion in a gene encoding a transcription factor) on cellulase production by constructing knock-out strains in the QM6a background. Neither the 85 kb deletion nor the deletion of the transcription factor affected cellulase production.
conclusionsaCGH analysis identified dozens of mutations in each strain analyzed. The resolution was at the level of single nucleotide mutation. High-density aCGH is a powerful tool for genome-wide analysis of organisms with small genomes e.g. fungi, especially in studies where a large set of interesting strains is analyzed.
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