ArticleThe Journal of membrane biology2010
The role of electrophoresis in gene electrotransfer.
Article in The Journal of membrane biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- Gold Nanoparticles-Enhanced Gene Transfer Driven by MHz-Frequency Nanosecond Pulsed Electric Fields.Biomolecules · 2025Article
- Feasibility Study for the Use of Gene Electrotransfer and Cell Electrofusion as a Single-Step Technique for the Generation of Activated Cancer Cell Vaccines.The Journal of membrane biology · 2024Article
- Importance of the electrophoresis and pulse energy for siRNA-mediated gene silencing by electroporation in differentiated primary human myotubes.Biomedical engineering online · 2024Article
- Improving NonViral Gene Delivery Using MHz Bursts of Nanosecond Pulses and Gold Nanoparticles for Electric Field Amplification.Pharmaceutics · 2023Article
- Gene Electrotransfer Efficiency in 2D and 3D Cancer Cell Models Using Different Electroporation Protocols: A Comparative Study.Pharmaceutics · 2023Article
- Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis.Pharmaceutics · 2022Review
- Advances of Electroporation-Related Therapies and the Synergy with Immunotherapy in Cancer Treatment.Vaccines · 2022Review
- Modeling the gene delivery process of the needle array-based tissue nanotransfection.Nano research · 2022Article
- Aluminum particles generated during millisecond electric pulse application enhance adenovirus-mediated gene transfer in L929 cells.Scientific reports · 2021Article
- Modelling FUS Mislocalisation in an In Vitro Model of Innervated Human Muscle.Journal of molecular neuroscience : MN · 2017Article
- Gene Electrotransfer: A Mechanistic Perspective.Current gene therapy · 2016Review
- Electrotransfection and lipofection show comparable efficiency for in vitro gene delivery of primary human myoblasts.The Journal of membrane biology · 2015Article
- New insights into the mechanisms of gene electrotransfer--experimental and theoretical analysis.Scientific reports · 2015Article
- Comparison of flow cytometry, fluorescence microscopy and spectrofluorometry for analysis of gene electrotransfer efficiency.The Journal of membrane biology · 2014Article
- Physical methods for intracellular delivery: practical aspects from laboratory use to industrial-scale processing.Journal of laboratory automation · 2014Review
- The impact of non-electrical factors on electrical gene transfer.Methods in molecular biology (Clifton, N.J.) · 2014Review
- Optimization of The Electroporation Conditions for Transfection of Human Factor IX into The Goat Fetal Fibroblasts.Cell journal · 2013Article
- Electric field orientation for gene delivery using high-voltage and low-voltage pulses.The Journal of membrane biology · 2012Article
- Changing the direction and orientation of electric field during electric pulses application improves plasmid gene transfer in vitro.Journal of visualized experiments : JoVE · 2011Article
- Numerical optimization of gene electrotransfer into muscle tissue.Biomedical engineering online · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene electrotransfer is an established method for gene delivery which uses high-voltage pulses to increase the permeability of a cell membrane and enables transfer of genes. Poor plasmid mobility in tissues is one of the major barriers for the successful use of gene electrotransfer in gene therapy. Therefore, we analyzed the effect of electrophoresis on increasing gene electrotransfer efficiency using different combinations of high-voltage (HV) and low-voltage (LV) pulses in vitro on CHO cells. We designed a special prototype of electroporator, which enabled us to use only HV pulses or combinations of LV + HV and HV + LV pulses. We used optimal plasmid concentrations used in in vitro conditions as well as lower suboptimal concentrations in order to mimic in vivo conditions. Only for the lowest plasmid concentration did the electrophoretic force of the LV pulse added to the HV pulse increase the transfection efficiency compared to using only HV. The effect of the LV pulse was more pronounced for HV + LV, while for the reversed sequence, LV + HV, there was only a minor effect of the LV pulse. For the highest plasmid concentrations no added effect of LV pulses were observed. Our results suggest that there are different contributing effects of LV pulses: electrophoretically increased contact of DNA with the membrane and increased insertion of DNA into permeabilized cell membrane and/or translocation due to electrophoretic force, which appears to be the dominant effect.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.