ArticleNature structural & molecular biology2010
Replication stress checkpoint signaling controls tRNA gene transcription.
Article in Nature structural & molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 45 citations in OpenAlex.
- Review
- Review
- Hydroxyurea and inactivation of checkpoint kinase MEC1 inhibit transcription termination and pre-mRNA cleavage at polyadenylation sites in budding yeast.Scientific reports · 2023Article
- A regulatory phosphorylation site on Mec1 controls chromatin occupancy of RNA polymerases during replication stress.The EMBO journal · 2021Article
- Topoisomerase 1 prevents replication stress at R-loop-enriched transcription termination sites.Nature communications · 2020Article
- Maf1-dependent transcriptional regulation of tRNAs prevents genomic instability and is associated with extended lifespan.Aging cell · 2020Article
- Yeast PAF1 complex counters the pol III accumulation and replication stress on the tRNA genes.Scientific reports · 2019Article
- Mechanisms of Oncogene-Induced Replication Stress: Jigsaw Falling into Place.Cancer discovery · 2018Review
- The La and related RNA-binding proteins (LARPs): structures, functions, and evolving perspectives.Wiley interdisciplinary reviews. RNA · 2017Review
- Factors That Shape Eukaryotic tRNAomes: Processing, Modification and Anticodon-Codon Use.Biomolecules · 2017Review
- Review
- Transcription-replication conflicts: how they occur and how they are resolved.Nature reviews. Molecular cell biology · 2016Review
- RNA Polymerase III Advances: Structural and tRNA Functional Views.Trends in biochemical sciences · 2016Review
- Mec1, INO80, and the PAF1 complex cooperate to limit transcription replication conflicts through RNAPII removal during replication stress.Genes & development · 2016Article
- tRNA processing defects induce replication stress and Chk2-dependent disruption of piRNA transcription.The EMBO journal · 2015Article
- A fluorescent bimolecular complementation screen reveals MAF1, RNF7 and SETD3 as PCNA-associated proteins in human cells.Cell cycle (Georgetown, Tex.) · 2015Article
- Argonaute 2 Binds Directly to tRNA Genes and Promotes Gene Repression in cis.Molecular and cellular biology · 2015Article
- Replication and transcription on a collision course: eukaryotic regulation mechanisms and implications for DNA stability.Frontiers in genetics · 2015Review
- Coordination of tRNA transcription with export at nuclear pore complexes in budding yeast.Genes & development · 2014Article
- Regulation of cell death by transfer RNA.Antioxidants & redox signaling · 2013Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In budding yeast, the transcriptional machinery at tRNA genes naturally interferes with replication in a way that can promote chromosome breakage. Here we show that a signaling module composed of core components of the replication stress checkpoint pathway represses this fork-pausing machinery in normally cycling and genotoxin-treated cells. Specifically, the sensor kinase Mec1, the signaling adaptor Mrc1 and the transducer kinase Rad53 relay signals that globally repress tRNA gene transcription during unchallenged proliferation and under conditions of replication stress. Repressive signaling in genotoxin-treated cells requires Rad53-dependent activation of a conserved repressor of tRNA gene transcription, Maf1. Cells lacking Maf1 are sensitive to replication stress under conditions of elevated tRNA gene transcription. We propose that checkpoint control of the fork-pausing activity of tRNA genes complements the repertoire of replisome-targeted mechanisms by which checkpoint proteins promote faithful DNA replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.