ArticleNature methods2010
Amplification-free digital gene expression profiling from minute cell quantities.
Article in Nature methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Systematic detection of mCell reports methods · 2021Article
- Splicing heterogeneity: separating signal from noise.Genome biology · 2018Article
- Cell type-specific transcriptome profiling in mammalian brains.Frontiers in bioscience (Landmark edition) · 2016Review
- Integrating ChIP-sequencing and digital gene expression profiling to identify BRD7 downstream genes and construct their regulating network.Molecular and cellular biochemistry · 2016Article
- TREM2 and the neuroimmunology of Alzheimer's disease.Biochemical pharmacology · 2014Review
- The microglial sensome revealed by direct RNA sequencing.Nature neuroscience · 2013Article
- DNA sequencing methods in human genetics and disease research.F1000prime reports · 2013Review
- Direct RNA sequencing mediated identification of mRNA localized in protrusions of human MDA-MB-231 metastatic breast cancer cells.Journal of molecular signaling · 2013Article
- Population transcriptomics with single-cell resolution: a new field made possible by microfluidics: a technology for high throughput transcript counting and data-driven definition of cell types.BioEssays : news and reviews in molecular, cellular and developmental biology · 2013Article
- Circulating breast tumor cells exhibit dynamic changes in epithelial and mesenchymal composition.Science (New York, N.Y.) · 2013Article
- Identifying individual DNA species in a complex mixture by precisely measuring the spacing between nicking restriction enzymes with atomic force microscope.Journal of the Royal Society, Interface · 2012Article
- Dual RNA-seq of pathogen and host.Nature reviews. Microbiology · 2012Review
- RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis.Nature · 2012Article
- Single-cell and regional gene expression analysis in Alzheimer's disease.Cellular and molecular neurobiology · 2012Review
- On chromatin remodeling in mammary gland differentiation and breast tumorigenesis.Cold Spring Harbor perspectives in biology · 2012Review
- Third-generation sequencing techniques and applications to drug discovery.Expert opinion on drug discovery · 2012Review
- Digital RNA sequencing minimizes sequence-dependent bias and amplification noise with optimized single-molecule barcodes.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- SNP Discovery through Next-Generation Sequencing and Its Applications.International journal of plant genomics · 2012Article
- Transcriptomic analysis of rice (Oryza sativa) developing embryos using the RNA-Seq technique.PloS one · 2012Article
- Counting absolute numbers of molecules using unique molecular identifiers.Nature methods · 2011Article
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9 authors.
Funding
Abstract
Generating reliable expression profiles from minute cell quantities is critical for scientific discovery and potential clinical applications. Here we present low-quantity digital gene expression (LQ-DGE), an amplification-free approach involving capture of poly(A)(+) RNAs from cellular lysates onto poly(dT)-coated sequencing surfaces, followed by on-surface reverse transcription and sequencing. We applied LQ-DGE to profile malignant and nonmalignant mouse and human cells, demonstrating its quantitative power and potential applicability to archival specimens.
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