ArticleThe Journal of biological chemistry2010
The expression of aquaporin-1 in the medulla of the kidney is dependent on the transcription factor associated with hypertonicity, TonEBP.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 56 citations in OpenAlex.
- Dehydration promotes intracellular lipid synthesis and accumulation.Nature communications · 2026Article
- NFAT5: a stress-related transcription factor with multiple functions in health and disease.Cell stress · 2025Review
- Inhibition of NFAT5-Dependent Astrocyte Swelling Alleviates Neuropathic Pain.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Investigating post-traumatic syringomyelia and local fluid osmoregulation via a rat model.Fluids and barriers of the CNS · 2024Article
- Autophagy is involved in degradation of AQP1 in response to an acute decrement in tonicity.iScience · 2023Article
- Review
- The old and the new about the contractile vacuole of Trypanosoma cruzi.The Journal of eukaryotic microbiology · 2022Review
- TonEBP regulates the hyperosmotic expression of aquaporin 1 and 5 in the intervertebral disc.Scientific reports · 2021Article
- NFAT5, which protects against hypertonicity, is activated by that stress via structuring of its intrinsically disordered domain.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Potential Role of Gene Regulator NFAT5 in the Pathogenesis of Diabetes Mellitus.Journal of diabetes research · 2020Review
- Article
- Uric acid activates aldose reductase and the polyol pathway for endogenous fructose and fat production causing development of fatty liver in rats.The Journal of biological chemistry · 2019Article
- Identification of NFAT5 as a transcriptional regulator of the EDN1 gene in collecting duct.American journal of physiology. Renal physiology · 2019Article
- NFAT5 up-regulates expression of the kidney-specific ubiquitin ligase geneThe Journal of biological chemistry · 2019Article
- Downregulation of the S1P Transporter Spinster Homology Protein 2 (Spns2) Exerts an Anti-Fibrotic and Anti-Inflammatory Effect in Human Renal Proximal Tubular Epithelial Cells.International journal of molecular sciences · 2018Article
- High salt intake causes leptin resistance and obesity in mice by stimulating endogenous fructose production and metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- pH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor.BMC molecular biology · 2018Article
- Mouse Metanephric Mesenchymal Cell-Derived Angioblasts Undergo Vasculogenesis in Three-Dimensional Culture.The American journal of pathology · 2018Article
- Hyperosmotic stress stimulates autophagy via polycystin-2.Oncotarget · 2017Article
- Protective role of fructokinase blockade in the pathogenesis of acute kidney injury in mice.Nature communications · 2017Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of aquaporin-1 (AQP1) and -2 (AQP2) channels in the kidney are critical for the maintenance of water homeostasis and the operation of the urinary concentrating mechanism. Hypertonic stress induced in inner medullary (IMCD3) cells by addition of NaCl to the medium substantially up-regulated the mRNA and protein expression of AQP1, suggesting that its activation occurs at a transcriptional and a translational levels. In contrast, no up-regulation of AQP1 was observed when these cells were exposed to the same tonicity by addition of urea. To explore the transcriptional activation of aqp1 under hypertonic stress, we examined the role of the transcription factor associated with hypertonicity, TonEBP. Treatment of IMCD3 cells with the TonEBP inhibitor rottlerin or silencing its expression with specific shRNA technology led to a substantial reduction in AQP1 expression under hypertonic conditions. Moreover, we defined a conserved TonEBP binding site located 811 bp upstream of the aqp1 exon that is essential for its expression. Single site-directed mutation of this TonE site led to a 54 ± 5% (p < 0.01) decrease in AQP1 luciferase-driven activity under hypertonic stress. TonEBP mutant mice display marked decrement in the expression of AQP1 in the inner medulla. In conclusion, these data demonstrate that TonEBP is necessary for the regulation of AQP1 expression in the inner medulla of the kidney under hypertonic conditions.
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