ArticleAmerican journal of respiratory and critical care medicine2010
Alveolar epithelial cell injury due to zinc oxide nanoparticle exposure.
Article in American journal of respiratory and critical care medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed, 102 citations in OpenAlex.
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- Polymeric nanoparticle-mediated siRNA delivery into primary human bronchial airway cells.American journal of physiology. Cell physiology · 2026Article
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- ZnO nanoparticles induce acute arrhythmia and heart failure in mice by disturbing cardiac ion channels.Frontiers in cardiovascular medicine · 2025Article
- Zinc Toxicity: Understanding the Limits.Molecules (Basel, Switzerland) · 2024Review
- Therapeutic Approaches for the Treatment of Interstitial Lung Disease: An Exploratory Review on Molecular Mechanisms.Mini reviews in medicinal chemistry · 2024Review
- Investigating the Effects of Biogenic Zinc Oxide Nanoparticles Produced Using Papaver somniferum Extract on Oxidative Stress, Cytotoxicity, and the Induction of Apoptosis in the THP-1 Cell Line.Biological trace element research · 2023Article
- Determinants and mechanisms of inorganic nanoparticle translocation across mammalian biological barriers.Archives of toxicology · 2023Review
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- A multiplex inhalation platform to modelFrontiers in pharmacology · 2023Article
- Strategies to improve bioactive and antibacterial properties of polyetheretherketone (PEEK) for use as orthopedic implants.Materials today. Bio · 2022Review
- Chemistry and lung toxicity of particulate matter emitted from firearms.Scientific reports · 2022Article
- Nanoparticles as a Tool in Neuro-Oncology Theranostics.Pharmaceutics · 2021Review
- Reactive Oxygen Species-Related Nanoparticle Toxicity in the Biomedical Field.Nanoscale research letters · 2020Review
- Oxidative stress mediated hepatotoxicity induced by ZNP and modulatory role of fruit extract on male Wistar rat.Toxicology reports · 2020Article
- The pro-inflammatory stimulus of zinc- and copper-containing welding fumes in whole blood assay via protein tyrosine phosphatase 1B inhibition.Scientific reports · 2019Article
- Antileishmanial activity and cytotoxicity of ZnO-based nano-formulations.International journal of nanomedicine · 2019Article
- Alveolar epithelial cell processing of nanoparticles activates autophagy and lysosomal exocytosis.American journal of physiology. Lung cellular and molecular physiology · 2018Article
- Differential Contribution of Constituent Metal Ions to the Cytotoxic Effects of Fast-Dissolving Metal-Oxide Nanoparticles.Frontiers in pharmacology · 2018Article
- Mitochondrial Dysfunction in Pulmonary Fibrosis.Annals of the American Thoracic Society · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
rationaleAlthough inhalation of zinc oxide (ZnO) nanoparticles (NPs) is known to cause systemic disease (i.e., metal fume fever), little is known about mechanisms underlying injury to alveolar epithelium.
objectivesInvestigate ZnO NP-induced injury to alveolar epithelium by exposing primary cultured rat alveolar epithelial cell monolayers (RAECMs) to ZnO NPs.
methodsRAECMs were exposed apically to ZnO NPs or, in some experiments, to culture fluid containing ZnCl₂ or free Zn released from ZnO NPs. Transepithelial electrical resistance (R(T)) and equivalent short-circuit current (I(EQ)) were assessed as functions of concentration and time. Morphologic changes, lactate dehydrogenase release, cell membrane integrity, intracellular reactive oxygen species (ROS), and mitochondrial activity were measured. MEASUREMENTS AND MAIN
resultsApical exposure to 176 μg/ml ZnO NPs decreased R(T) and I(EQ) of RAECMs by 100% over 24 hours, whereas exposure to 11 μg/ml ZnO NPs had little effect. Changes in R(T) and I(EQ) caused by 176 μg/ml ZnO NPs were irreversible. ZnO NP effects on R(T) yielded half-maximal concentrations of approximately 20 μg/ml. Apical exposure for 24 hours to 176 μg/ml ZnO NPs induced decreases in mitochondrial activity and increases in lactate dehydrogenase release, permeability to fluorescein sulfonic acid, increased intracellular ROS, and translocation of ZnO NPs from apical to basolateral fluid (most likely across injured cells and/or damaged paracellular pathways).
conclusionsZnO NPs cause severe injury to RAECMs in a dose- and time-dependent manner, mediated, at least in part, by free Zn released from ZnO NPs, mitochondrial dysfunction, and increased intracellular ROS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.