ArticleJournal of bacteriology2010
Pmr, a histone-like protein H1 (H-NS) family protein encoded by the IncP-7 plasmid pCAR1, is a key global regulator that alters host function.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- Structural characterization of the native oligomerization mode of MvaT proteins inMicrobiology spectrum · 2026Article
- The Ros/MucR Zinc-Finger Protein Family in Bacteria: Structure and Functions.International journal of molecular sciences · 2022Review
- Why do plasmids manipulate the expression of bacterial phenotypes?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- A Novel Gene Cluster Is Involved in the Degradation of Lignin-Derived Monoaromatics in Thermus oshimai JL-2.Applied and environmental microbiology · 2021Article
- Review
- Ancestral zinc-finger bearing protein MucR in alpha-proteobacteria: A novel xenogeneic silencer?Computational and structural biotechnology journal · 2020Review
- H-NS Family Proteins Drastically Change Their Targets in Response to the Horizontal Transfer of the Catabolic Plasmid pCAR1.Frontiers in microbiology · 2020Article
- Impact of Xenogeneic Silencing on Phage-Host Interactions.Journal of molecular biology · 2019Review
- Endogenous and Foreign Nucleoid-Associated Proteins of Bacteria: Occurrence, Interactions and Effects on Mobile Genetic Elements and Host's Biology.Computational and structural biotechnology journal · 2019Review
- Reconsidering plasmid maintenance factors for computational plasmid design.Computational and structural biotechnology journal · 2019Review
- Growth phase-dependent expression profiles of three vital H-NS family proteins encoded on the chromosome of Pseudomonas putida KT2440 and on the pCAR1 plasmid.BMC microbiology · 2017Article
- MvaT Family Proteins Encoded on IncP-7 Plasmid pCAR1 and the Host Chromosome Regulate the Host Transcriptome Cooperatively but Differently.Applied and environmental microbiology · 2016Article
- Effects of three different nucleoid-associated proteins encoded on IncP-7 plasmid pCAR1 on host Pseudomonas putida KT2440.Applied and environmental microbiology · 2015Article
- Positive epistasis between co-infecting plasmids promotes plasmid survival in bacterial populations.The ISME journal · 2014Article
- Article
- Antibiotic resistance shaping multi-level population biology of bacteria.Frontiers in microbiology · 2013Article
- Transcriptome mapping of pAR060302, a blaCMY-2-positive broad-host-range IncA/C plasmid.Applied and environmental microbiology · 2012Article
- Evolution of genome base composition and genome size in bacteria.Frontiers in microbiology · 2012Article
- IncA/C plasmids: An emerging threat to human and animal health?Mobile genetic elements · 2012Article
- Distribution of genes encoding nucleoid-associated protein homologs in plasmids.International journal of evolutionary biology · 2011Article
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Histone-like protein H1 (H-NS) family proteins are nucleoid-associated proteins (NAPs) conserved among many bacterial species. The IncP-7 plasmid pCAR1 is transmissible among various Pseudomonas strains and carries a gene encoding the H-NS family protein, Pmr. Pseudomonas putida KT2440 is a host of pCAR1, which harbors five genes encoding the H-NS family proteins PP_1366 (TurA), PP_3765 (TurB), PP_0017 (TurC), PP_3693 (TurD), and PP_2947 (TurE). Quantitative reverse transcription-PCR (qRT-PCR) demonstrated that the presence of pCAR1 does not affect the transcription of these five genes and that only pmr, turA, and turB were primarily transcribed in KT2440(pCAR1). In vitro pull-down assays revealed that Pmr strongly interacted with itself and with TurA, TurB, and TurE. Transcriptome comparisons of the pmr disruptant, KT2440, and KT2440(pCAR1) strains indicated that pmr disruption had greater effects on the host transcriptome than did pCAR1 carriage. The transcriptional levels of some genes that increased with pCAR1 carriage, such as the mexEF-oprN efflux pump genes and parI, reverted with pmr disruption to levels in pCAR1-free KT2440. Transcriptional levels of putative horizontally acquired host genes were not altered by pCAR1 carriage but were altered by pmr disruption. Identification of genome-wide Pmr binding sites by ChAP-chip (chromatin affinity purification coupled with high-density tiling chip) analysis demonstrated that Pmr preferentially binds to horizontally acquired DNA regions. The Pmr binding sites overlapped well with the location of the genes differentially transcribed following pmr disruption on both the plasmid and the chromosome. Our findings indicate that Pmr is a key factor in optimizing gene transcription on pCAR1 and the host chromosome.
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