ArticleJournal of bacteriology2010
Expressed genome of Methylobacillus flagellatus as defined through comprehensive proteomics and new insights into methylotrophy.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 36 citations in OpenAlex.
- XoxF and the Calvin-Benson cycle mediate lanthanide-dependent growth on methanol inApplied and environmental microbiology · 2025Article
- A synthetic methylotrophicNature catalysis · 2024Article
- GroEL-Proteotyping of Bacterial Communities Using Tandem Mass Spectrometry.International journal of molecular sciences · 2023Article
- Synergistic investigation of natural and synthetic C1-trophic microorganisms to foster a circular carbon economy.Nature communications · 2023Review
- Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy.Microorganisms · 2022Review
- Enzyme engineering andSynthetic biology (Oxford, England) · 2021Article
- The expanded diversity of methylophilaceae from Lake Washington through cultivation and genomic sequencing of novel ecotypes.PloS one · 2014Article
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- Article
- Stimulation of methanotrophic growth in cocultures by cobalamin excreted by rhizobia.Applied and environmental microbiology · 2011Article
- An integrated proteomics/transcriptomics approach points to oxygen as the main electron sink for methanol metabolism in Methylotenera mobilis.Journal of bacteriology · 2011Article
- Genomes of three methylotrophs from a single niche reveal the genetic and metabolic divergence of the methylophilaceae.Journal of bacteriology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, techniques have been developed and perfected for high-throughput identification of proteins and their accurate partial sequencing by shotgun nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS), making it feasible to assess global protein expression profiles in organisms with sequenced genomes. We implemented comprehensive proteomics to assess the expressed portion of the genome of Methylobacillus flagellatus during methylotrophic growth. We detected a total of 1,671 proteins (64% of the inferred proteome), including all the predicted essential proteins. Nonrandom patterns observed with the nondetectable proteins appeared to correspond to silent genomic islands, as inferred through functional profiling and genome localization. The protein contents in methylamine- and methanol-grown cells showed a significant overlap, confirming the commonality of methylotrophic metabolism downstream of the primary oxidation reactions. The new insights into methylotrophy include detection of proteins for the N-methylglutamate methylamine oxidation pathway that appears to be auxiliary and detection of two alternative enzymes for both the 6-phosphogluconate dehydrogenase reaction (GndA and GndB) and the formate dehydrogenase reaction (FDH1 and FDH4). Mutant analysis revealed that GndA and FDH4 are crucial for the organism's fitness, while GndB and FDH1 are auxiliary.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.