ArticleBiochimica et biophysica acta2010
Discrimination between two possible reaction sequences that create potential risk of generation of deleterious radicals by cytochrome bc₁. Implications for the mechanism of superoxide production.
Article in Biochimica et biophysica acta, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
- Recent insights into the molecular mechanism of ubiquinol oxidation by cytochrome bcFEBS open bio · 2026Review
- Mitochondrial Cristae Morphology Reflecting Metabolism, Superoxide Formation, Redox Homeostasis, and Pathology.Antioxidants & redox signaling · 2023Review
- Unexpected Heme Redox Potential Values Implicate an Uphill Step in CytochromeThe journal of physical chemistry. B · 2022Article
- Superoxide dismutase 2 ameliorates mitochondrial dysfunction in skin fibroblasts of Leber's hereditary optic neuropathy patients.Frontiers in neuroscience · 2022Article
- Molecular and Supramolecular Structure of the Mitochondrial Oxidative Phosphorylation System: Implications for Pathology.Life (Basel, Switzerland) · 2021Review
- Catalytic Reactions and Energy Conservation in the CytochromeChemical reviews · 2021Review
- The High-Spin HemeFrontiers in chemistry · 2021Article
- Modeling the Energy Landscape of Side Reactions in the Cytochrome bcFrontiers in chemistry · 2021Article
- Sodium sulfide selectively induces oxidative stress, DNA damage, and mitochondrial dysfunction and radiosensitizes glioblastoma (GBM) cells.Redox biology · 2019Article
- The antimicrobial activity of chemerin-derived peptide p4 requires oxidative conditions.The Journal of biological chemistry · 2019Article
- Mitochondria as Potential Targets and Initiators of the Blue Light Hazard to the Retina.Oxidative medicine and cellular longevity · 2019Review
- Review
- Natively oxidized amino acid residues in the spinach cytochrome bPhotosynthesis research · 2018Article
- Analysis of a Functional Dimer Model of Ubiquinol Cytochrome c Oxidoreductase.Biophysical journal · 2017Article
- Metastable radical state, nonreactive with oxygen, is inherent to catalysis by respiratory and photosynthetic cytochromes bc1/b6f.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Mitochondrial disease-related mutations at the cytochrome b-iron-sulfur protein (ISP) interface: Molecular effects on the large-scale motion of ISP and superoxide generation studied in Rhodobacter capsulatus cytochrome bc1.Biochimica et biophysica acta · 2016Article
- Distinct properties of semiquinone species detected at the ubiquinol oxidation Qo site of cytochrome bc1 and their mechanistic implications.Journal of the Royal Society, Interface · 2016Review
- Effect of H bond removal and changes in the position of the iron-sulphur head domain on the spin-lattice relaxation properties of the [2Fe-2S](2+) Rieske cluster in cytochrome bc(1).Physical chemistry chemical physics : PCCP · 2015Article
- Mitochondrial Disease-related Mutation G167P in Cytochrome b of Rhodobacter capsulatus Cytochrome bc1 (S151P in Human) Affects the Equilibrium Distribution of [2Fe-2S] Cluster and Generation of Superoxide.The Journal of biological chemistry · 2015Article
- Electronic connection between the quinone and cytochrome C redox pools and its role in regulation of mitochondrial electron transport and redox signaling.Physiological reviews · 2015Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In addition to its bioenergetic function of building up proton motive force, cytochrome bc₁ can be a source of superoxide. One-electron reduction of oxygen is believed to occur from semiquinone (SQ(o)) formed at the quinone oxidation/reduction Q(o) site (Q(o)) as a result of single-electron oxidation of quinol by the iron-sulfur cluster (FeS) (semiforward mechanism) or single-electron reduction of quinone by heme b(L) (semireverse mechanism). It is hotly debated which mechanism plays a major role in the overall production of superoxide as experimental data supporting either reaction exist. To evaluate a contribution of each of the mechanisms we first measured superoxide production under a broad range of conditions using the mutants of cytochrome bc₁ that severely impeded the oxidation of FeS by cytochrome c₁, changed density of FeS around Q(o) by interfering with its movement, or combined these two effects together. We then compared the amount of generated superoxide with mathematical models describing either semiforward or semireverse mechanism framed within a scheme assuming competition between the internal reactions at Q(o) and the leakage of electrons on oxygen. We found that only the model of semireverse mechanism correctly reproduced the experimentally measured decrease in ROS for the FeS motion mutants and increase in ROS for the mutants with oxidation of FeS impaired. This strongly suggests that this mechanism dominates in setting steady-state levels of SQ(o) that present a risk of generation of superoxide by cytochrome bc₁. Isolation of this reaction sequence from multiplicity of possible reactions at Q(o) helps to better understand conditions under which complex III might contribute to ROS generation in vivo.
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