ArticleCellular microbiology2010
The stage-regulated HASPB and SHERP proteins are essential for differentiation of the protozoan parasite Leishmania major in its sand fly vector, Phlebotomus papatasi.
Article in Cellular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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70 citing papers in PubMed, 103 citations in OpenAlex.
- Identification of transporters essential for survival of Leishmania promastigotes in the digestive tract of sand flies.PLoS pathogens · 2026Article
- First experimental assessment of Phlebotomus mascittii vector competence for Leishmania infantum and Leishmania martiniquensis.Parasites & vectors · 2026Article
- Ecdysone signaling acts as a regulator of immunity and microbiota balance, shapingFrontiers in immunology · 2026Article
- Impact of Asaia bacteria on Leishmania major development in sand flies: implications for vector control strategies.Parasites & vectors · 2025Article
- Gene deletion as a possible strategy adopted by New World Leishmania infantum to maximize geographic dispersion.PLoS pathogens · 2025Article
- Detection of Leishmania metacyclogenesis within the sand fly vector employing a real-time PCR for sherp gene expression: A tool for Leishmania surveillance and transmission potential.PLoS neglected tropical diseases · 2025Article
- Single-cell atlas ofProceedings of the National Academy of Sciences of the United States of America · 2024Article
- CYP5122A1 encodes an essential sterol C4-methyl oxidase in Leishmania donovani and determines the antileishmanial activity of antifungal azoles.Nature communications · 2024Article
- The development of L. major, L. donovani and L. martiniquensis, Leishmania currently emerging in Europe, in the sand fly species Phlebotomus perniciosus and P. tobbi.PLoS neglected tropical diseases · 2024Article
- Discovery of essential kinetoplastid-insect adhesion proteins and their function in Leishmania-sand fly interactions.Nature communications · 2024Article
- Whole cell reconstructions of Leishmania mexicana through the cell cycle.PLoS pathogens · 2024Article
- Comparative genomics of Leishmania donovani progeny from genetic crosses in two sand fly species and impact on the diversity of diagnostic and vaccine candidates.PLoS neglected tropical diseases · 2024Article
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- Evidence of a conserved mammalian immunosuppression mechanism inFrontiers in immunology · 2023Article
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- RNAi-mediated gene silencing ofFrontiers in physiology · 2023Article
- Expanded Proteomic Survey of the Human Parasite Leishmania major Focusing on Changes in Null Mutants of the Golgi GDP-Mannose/Fucose/Arabinopyranose TransporterMicrobiology spectrum · 2022Article
- Review
- Linear and conformational determinants of visceral leishmaniasis diagnostic antigens rK28 and rK39.Parasites & vectors · 2022Article
10 more citing papers are in PubMed but not listed here.
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6 authors at 2 institutions in 1 country.
Funding
Abstract
The stage-regulated HASPB and SHERP proteins of Leishmania major are predominantly expressed in cultured metacyclic parasites that are competent for macrophage uptake and survival. The role of these proteins in parasite development in the sand fly vector has not been explored, however. Here, we confirm that expression of HASPB is detected only in vector metacyclic stages, correlating with the expression of metacyclic-specific lipophosphoglycan and providing the first definitive protein marker for this infective sand fly stage. Similarly, SHERP is expressed in vector metacyclics but is also detected at low levels in the preceding short promastigote stage. Using genetically modified parasites lacking or complemented for the LmcDNA16 locus on chromosome 23 that contains the HASP and SHERP genes, we further show that the presence of this locus is essential for parasite differentiation to the metacyclic stage in Phlebotomus papatasi. While wild-type and complemented parasites transform normally in late-stage infections, generating metacyclic promastigotes and colonizing the sand fly stomodeal valve, null parasites accumulate at the earlier elongated nectomonad stage of development within the abdominal and thoracic midgut of the sand fly. Complementation with HASPB or SHERP alone suggests that HASPB is the dominant effector molecule in this process.
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