ArticleJournal of cellular and molecular medicine2011
Directly auto-transplanted mesenchymal stem cells induce bone formation in a ceramic bone substitute in an ectopic sheep model.
Article in Journal of cellular and molecular medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04001842 (Mandibular Reconstruction Using Tissue Regeneration With Axially Vascularised Bone Substitutes), which is not on this map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Mandibular Reconstruction Using Tissue Regeneration With Axially Vascularised Bone Substitutes
Who cites it
25 citing papers in PubMed, 56 citations in OpenAlex.
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- Why Animal Experiments Are Still Indispensable in Bone Research: A Statement by the European Calcified Tissue Society.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023Review
- Personalized medicine for reconstruction of critical-size bone defects - a translational approach with customizable vascularized bone tissue.NPJ Regenerative medicine · 2021Article
- Isolation and characterization of ovine umbilical cord-derived mesenchymal stem cells.Cytotechnology · 2019Article
- In vitro expansion impaired the stemness of early passage mesenchymal stem cells for treatment of cartilage defects.Cell death & disease · 2017Article
- Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering.PloS one · 2017Article
- Restoration of bone defects using modified heterogeneous deproteinized bone seeded with bone marrow mesenchymal stem cells.American journal of translational research · 2017Article
- Synergistic enhancement of ectopic bone formation by supplementation of freshly isolated marrow cells with purified MSC in collagen-chitosan hydrogel microbeads.Connective tissue research · 2016Article
- Combination of BMP2 and MSCs significantly increases bone formation in the rat arterio-venous loop model.Tissue engineering. Part A · 2015Article
- A hundred patients with vertical rectus abdominis myocutaneous (VRAM) flap for pelvic reconstruction after total pelvic exenteration.International journal of colorectal disease · 2014Article
- Successful human long-term application of in situ bone tissue engineering.Journal of cellular and molecular medicine · 2014Article
- Repair of segmental load-bearing bone defect by autologous mesenchymal stem cells and plasma-derived fibrin impregnated ceramic block results in early recovery of limb function.BioMed research international · 2014Article
- Comparison of uncultured marrow mononuclear cells and culture-expanded mesenchymal stem cells in 3D collagen-chitosan microbeads for orthopedic tissue engineering.Tissue engineering. Part A · 2014Article
- Comparison of the efficacy of bone marrow mononuclear cells and bone mesenchymal stem cells in the treatment of osteoarthritis in a sheep model.International journal of clinical and experimental pathology · 2014Article
- Evaluation of angiogenesis of bioactive glass in the arteriovenous loop model.Tissue engineering. Part C, Methods · 2013Article
- An anti-infection tissue-engineered construct delivering vancomycin: its evaluation in a goat model of femur defect.International journal of medical sciences · 2013Article
- Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC.Stem cells international · 2013Article
- [Pathogenesis, classification and diagnosis of necrotizing soft tissue infections].Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen · 2012Review
- Detecting of breast cancer metastasis by means of regional lymph node sampling during autologous breast reconstruction--a screening of 519 consecutive patients.Medical science monitor : international medical journal of experimental and clinical research · 2012Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bone tissue engineering approaches increasingly focus on the use of mesenchymal stem cells (MSC). In most animal transplantation models MSC are isolated and expanded before auto cell transplantation which might be critical for clinical application in the future. Hence this study compares the potential of directly auto-transplanted versus in vitro expanded MSC with or without bone morphogenetic protein-2 (BMP-2) to induce bone formation in a large volume ceramic bone substitute in the sheep model. MSC were isolated from bone marrow aspirates and directly auto-transplanted or expanded in vitro and characterized using fluorescence activated cell sorting (FACS) and RT-PCR analysis before subcutaneous implantation in combination with BMP-2 and β-tricalcium phosphate/hydroxyapatite (β-TCP/HA) granules. Constructs were explanted after 1 to 12 weeks followed by histological and RT-PCR evaluation. Sheep MSC were CD29(+), CD44(+) and CD166(+) after selection by Ficoll gradient centrifugation, while directly auto-transplanted MSC-populations expressed CD29 and CD166 at lower levels. Both, directly auto-transplanted and expanded MSC, were constantly proliferating and had a decreasing apoptosis over time in vivo. Directly auto-transplanted MSC led to de novo bone formation in a heterotopic sheep model using a β-TCP/HA matrix comparable to the application of 60 μg/ml BMP-2 only or implantation of expanded MSC. Bone matrix proteins were up-regulated in constructs following direct auto-transplantation and in expanded MSC as well as in BMP-2 constructs. Up-regulation was detected using immunohistology methods and RT-PCR. Dense vascularization was demonstrated by CD31 immunohistology staining in all three groups. Ectopic bone could be generated using directly auto-transplanted or expanded MSC with β-TCP/HA granules alone. Hence BMP-2 stimulation might become dispensable in the future, thus providing an attractive, clinically feasible approach to bone tissue engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.