ArticlePloS one2010
Gene expression analysis of forskolin treated basilar papillae identifies microRNA181a as a mediator of proliferation.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Evolutionary constraints impose multiple checkpoints that block mammalian sensory hair cell regeneration.Frontiers in cell and developmental biology · 2026Review
- miRNA and mRNA Profiling Links Connexin Deficiency to Deafness via Early Oxidative Damage in the MouseFrontiers in cell and developmental biology · 2020Article
- MicroRNAs and Presbycusis.Aging and disease · 2018Review
- microRNA-183 is Essential for Hair Cell Regeneration after Neomycin Injury in Zebrafish.Yonsei medical journal · 2018Article
- Insights into inner ear-specific gene regulation: Epigenetics and non-coding RNAs in inner ear development and regeneration.Seminars in cell & developmental biology · 2017Review
- In silico genome wide mining of conserved and novel miRNAs in the brain and pineal gland of Danio rerio using small RNA sequencing data.Genomics data · 2016Article
- MicroRNAs in sensorineural diseases of the ear.Frontiers in molecular neuroscience · 2013Review
- The Transcriptomics to Proteomics of Hair Cell Regeneration: Looking for a Hair Cell in a Haystack.Microarrays (Basel, Switzerland) · 2013Article
- Identifying microRNAs involved in degeneration of the organ of corti during age-related hearing loss.PloS one · 2013Article
- microRNAs: the art of silencing in the ear.EMBO molecular medicine · 2012Review
- MicroRNAs in inner ear biology and pathogenesis.Hearing research · 2012Review
- Gene expression gradients along the tonotopic axis of the chicken auditory epithelium.Journal of the Association for Research in Otolaryngology : JARO · 2011Article
- MicroRNA181a plays a key role in hair cell regeneration in the avian auditory epithelium.Neuroscience letters · 2011Article
- β4-subunit increases Slo responsiveness to physiological Ca2+ concentrations and together with β1 reduces surface expression of Slo in hair cells.American journal of physiology. Cell physiology · 2011Article
- Interactions between β-catenin and the HSlo potassium channel regulates HSlo surface expression.PloS one · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundAuditory hair cells spontaneously regenerate following injury in birds but not mammals. A better understanding of the molecular events underlying hair cell regeneration in birds may allow for identification and eventually manipulation of relevant pathways in mammals to stimulate regeneration and restore hearing in deaf patients. METHODOLOGY/PRINCIPAL
findingsGene expression was profiled in forskolin treated (i.e., proliferating) and quiescent control auditory epithelia of post-hatch chicks using an Affymetrix whole-genome chicken array after 24 (n = 6), 48 (n = 6), and 72 (n = 12) hours in culture. In the forskolin-treated epithelia there was significant (p<0.05; >two-fold change) upregulation of many genes thought to be relevant to cell cycle control and inner ear development. Gene set enrichment analysis was performed on the data and identified myriad microRNAs that are likely to be upregulated in the regenerating tissue, including microRNA181a (miR181a), which is known to mediate proliferation in other systems. Functional experiments showed that miR181a overexpression is sufficient to stimulate proliferation within the basilar papilla, as assayed by BrdU incorporation. Further, some of the newly produced cells express the early hair cell marker myosin VI, suggesting that miR181a transfection can result in the production of new hair cells. CONCLUSIONS/SIGNIFICANCE: These studies have identified a single microRNA, miR181a, that can cause proliferation in the chicken auditory epithelium with production of new hair cells.
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