ArticlePloS one2010
NELF potentiates gene transcription in the Drosophila embryo.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Regulation of RNA transcript elongation in metazoans and its relevance to disease.Nature reviews. Molecular cell biology · 2026Review
- NELF prevents transcriptional readthrough into DNA replication zones in cancer cells.EMBO reports · 2026Article
- eIF3i facilitates NELFCD translation to promote metastasis via regulating EMT and invadopodia.British journal of cancer · 2026Article
- Catalytic activity of the Bin3/MePCE methyltransferase domain is dispensable for 7SK snRNP function in Drosophila melanogaster.Genetics · 2024Article
- Transcriptional elongation control in developmental gene expression, aging, and disease.Molecular cell · 2023Review
- RNA Pol II pausing facilitates phased pluripotency transitions by buffering transcription.Genes & development · 2022Article
- Rapid and efficient degradation of endogenous proteins in vivo identifies stage-specific roles of RNA Pol II pausing in mammalian development.Developmental cell · 2022Article
- RNA polymerase II pausing in development: orchestrating transcription.Open biology · 2022Review
- NELF-A controls Drosophila healthspan by regulating heat-shock protein-mediated cellular protection and heterochromatin maintenance.Aging cell · 2021Article
- Poly(ADP-ribose) polymerase 1 in genome-wide expression control in Drosophila.Scientific reports · 2020Article
- The embryonic linker histone dBigH1 alters the functional state of active chromatin.Nucleic acids research · 2020Article
- Acute NelfA knockdown restricts compensatory gene expression and precipitates ventricular dysfunction during cardiac hypertrophy.Journal of molecular and cellular cardiology · 2020Article
- Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness inDevelopment (Cambridge, England) · 2018Article
- Article
- A balancing act: heterochromatin protein 1a and the Polycomb group coordinate their levels to silence chromatin in Drosophila.Epigenetics & chromatin · 2015Article
- Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B.PloS one · 2015Article
- Pausing for thought: disrupting the early transcription elongation checkpoint leads to developmental defects and tumourigenesis.BioEssays : news and reviews in molecular, cellular and developmental biology · 2013Article
- Extensive polymerase pausing during Drosophila axis patterning enables high-level and pliable transcription.Genes & development · 2013Article
- Pleiohomeotic interacts with the core transcription elongation factor Spt5 to regulate gene expression in Drosophila.PloS one · 2013Article
- Transcriptional pausing controls a rapid antiviral innate immune response in Drosophila.Cell host & microbe · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A hallmark of genes that are subject to developmental regulation of transcriptional elongation is association of the negative elongation factor NELF with the paused RNA polymerase complex. Here we use a combination of biochemical and genetic experiments to investigate the in vivo function of NELF in the Drosophila embryo. NELF associates with different gene promoter regions in correlation with the association of RNA polymerase II (Pol II) and the initial activation of gene expression during the early stages of embryogenesis. Genetic experiments reveal that maternally provided NELF is required for the activation, rather than the repression of reporter genes that emulate the expression of key developmental control genes. Furthermore, the relative requirement for NELF is dictated by attributes of the flanking cis-regulatory information. We propose that NELF-associated paused Pol II complexes provide a platform for high fidelity integration of the combinatorial spatial and temporal information that is central to the regulation of gene expression during animal development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.