ArticlePloS one2010
Single particle tracking of alpha7 nicotinic AChR in hippocampal neurons reveals regulated confinement at glutamatergic and GABAergic perisynaptic sites.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- Neuronal and Glial α7 Nicotinic Acetylcholine Receptors: Role in Alzheimer's Disease Pathophysiology.Life (Basel, Switzerland) · 2025Review
- Cholinergic Antagonists and Behavioral Disturbances in Neurodegenerative Diseases.International journal of molecular sciences · 2023Review
- Precise Detection and Visualization of Nanoscale Temporal Confinement in Single-Molecule Tracking Analysis.Membranes · 2022Article
- Neuronal Menin Overexpression Rescues Learning and Memory Phenotype in CA1-Specific α7 nAChRs KD Mice.Cells · 2021Article
- Nanoscale Sub-Compartmentalization of the Dendritic Spine Compartment.Biomolecules · 2021Review
- The HSPG syndecan is a core organizer of cholinergic synapses.The Journal of cell biology · 2021Article
- Regulation of membrane NMDA receptors by dynamics and protein interactions.The Journal of cell biology · 2021Review
- Light Sheet Illumination for 3D Single-Molecule Super-Resolution Imaging of Neuronal Synapses.Frontiers in synaptic neuroscience · 2021Review
- Detection of human neuronal α7 nicotinic acetylcholine receptors by conjugates of snake α-neurotoxin with quantum dots.Doklady. Biochemistry and biophysics · 2017Article
- Phospho-dependent Accumulation of GABABRs at Presynaptic Terminals after NMDAR Activation.Cell reports · 2016Article
- Motor and tail homology 1 (Th1) domains antagonistically control myosin-1 dynamics.Biophysical journal · 2014Article
- Detection, counting, and imaging of single nanoparticles.Analytical chemistry · 2014Review
- Diffusion dynamics of synaptic molecules during inhibitory postsynaptic plasticity.Frontiers in cellular neuroscience · 2014Review
- Presynaptic α7 nicotinic acetylcholine receptors enhance hippocampal mossy fiber glutamatergic transmission via PKA activation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Transient cholesterol effects on nicotinic acetylcholine receptor cell-surface mobility.PloS one · 2014Article
- Cell-surface translational dynamics of nicotinic acetylcholine receptors.Frontiers in synaptic neuroscience · 2014Review
- Visualization of lipid raft membrane compartmentalization in living RN46A neuronal cells using single quantum dot tracking.ACS chemical neuroscience · 2012Article
- PMCA2 via PSD-95 controls calcium signaling by α7-containing nicotinic acetylcholine receptors on aspiny interneurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Investigating the contribution of common genetic variants to the risk and pathogenesis of ADHD.The American journal of psychiatry · 2012Article
- The gain in brain: novel imaging techniques and multiplexed proteomic imaging of brain tissue ultrastructure.Current opinion in neurobiology · 2012Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alpha7 neuronal nicotinic acetylcholine receptors (alpha7-nAChR) form Ca(2+)-permeable homopentameric channels modulating cortical network activity and cognitive processing. They are located pre- and postsynaptically and are highly abundant in hippocampal GABAergic interneurons. It is unclear how alpha7-nAChRs are positioned in specific membrane microdomains, particularly in cultured neurons which are devoid of cholinergic synapses. To address this issue, we monitored by single particle tracking the lateral mobility of individual alpha7-nAChRs labeled with alpha-bungarotoxin linked to quantum dots in live rat cultured hippocampal interneurons. Quantitative analysis revealed different modes of lateral diffusion of alpha7-nAChR dependent on their subcellular localization. Confined receptors were found in the immediate vicinity of glutamatergic and GABAergic postsynaptic densities, as well as in extrasynaptic clusters of alpha-bungarotoxin labeling on dendrites. alpha7-nAChRs avoided entering postsynaptic densities, but exhibited reduced mobility and long dwell times at perisynaptic locations, indicative of regulated confinement. Their diffusion coefficient was lower, on average, at glutamatergic than at GABAergic perisynaptic sites, suggesting differential, synapse-specific tethering mechanisms. Disruption of the cytoskeleton affected alpha7-nAChR mobility and cell surface expression, but not their ability to form clusters. Finally, using tetrodotoxin to silence network activity, as well as exposure to a selective alpha7-nAChR agonist or antagonist, we observed that alpha7-nAChRs cell surface dynamics is modulated by chronic changes in neuronal activity. Altogether, given their high Ca(2+)-permeability, our results suggest a possible role of alpha7-nAChR on interneurons for activating Ca(2+)-dependent signaling in the vicinity of GABAergic and glutamatergic synapses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.