ArticleThe Journal of biological chemistry2010
Requirement of calcium binding, myristoylation, and protein-protein interaction for the Copine BON1 function in Arabidopsis.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- A natural allele of TaSCE1-A1 confers drought resistance in wheat.Nature plants · 2026Article
- Evolutionarily conserved BON1 regulates the basal cytosolic CaProceedings of the National Academy of Sciences of the United States of America · 2025Article
- TheFrontiers in plant science · 2025Article
- Pm57 from Aegilops searsii encodes a tandem kinase protein and confers wheat powdery mildew resistance.Nature communications · 2024Article
- Copine proteins are required for brassinosteroid signaling in maize and Arabidopsis.Nature communications · 2024Article
- Review
- Rice copine genes OsBON1 and OsBON3 function as suppressors of broad-spectrum disease resistance.Plant biotechnology journal · 2018Article
- Targeted Profiling ofThe Plant cell · 2018Article
- Calcium Pumps and Interacting BON1 Protein Modulate Calcium Signature, Stomatal Closure, and Plant Immunity.Plant physiology · 2017Article
- Opposing Effects on Two Phases of Defense Responses from Concerted Actions of HEAT SHOCK COGNATE70 and BONZAI1 in Arabidopsis.Plant physiology · 2015Article
- To die or not to die? Lessons from lesion mimic mutants.Frontiers in plant science · 2015Review
- The role of lipid post-translational modification in plant developmental processes.Frontiers in plant science · 2014Review
- The myristoylated amino-terminus of an Arabidopsis calcium-dependent protein kinase mediates plasma membrane localization.Plant molecular biology · 2013Article
- Disease resistance gene-induced growth inhibition is enhanced by rcd1 independent of defense activation in Arabidopsis.Plant physiology · 2013Article
- CPNA-1, a copine domain protein, is located at integrin adhesion sites and is required for myofilament stability in Caenorhabditis elegans.Molecular biology of the cell · 2013Article
- Article
- Complex regulation of an R gene SNC1 revealed by auto-immune mutants.Plant signaling & behavior · 2012Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Copines are highly conserved proteins with lipid-binding activities found in animals, plants, and protists. They contain two calcium-dependent phospholipid binding C2 domains at the amino terminus and a VWA domain at the carboxyl terminus. The biological roles of most copines are not understood and the biochemical properties required for their functions are largely unknown. The Arabidopsis copine gene BON1/CPN1 is a negative regulator of cell death and defense responses. Here we probed the potential biochemical activities of BON1 through mutagenic studies. We found that mutations of aspartates in the C2 domains did not alter plasma membrane localization but compromised BON1 activity. Mutation at putative myristoylation residue glycine 2 altered plasma membrane localization of BON1 and rendered BON1 inactive. Mass spectrometry analysis of BON1 further suggests that the N-peptide of BON1 is modified. Furthermore, mutations that affect the interaction between BON1 and its functional partner BAP1 abolished BON1 function. This analysis reveals an unanticipated regulation of copine protein localization and function by calcium and lipid modification and suggests an important role in protein-protein interaction for the VWA domain of copines.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.