ArticleMycopathologia2011
Characterization of recombinant terrelysin, a hemolysin of Aspergillus terreus.
Article in Mycopathologia, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Accelerating the understanding ofCurrent research in microbial sciences · 2024Review
- Towards Understanding the Function of Aegerolysins.Toxins · 2022Review
- Towards a Fungal Science That Is Independent of Researchers' Gender.Journal of fungi (Basel, Switzerland) · 2022Article
- Functional characterization of the AGL1 aegerolysin in the mycoparasitic fungus Trichoderma atroviride reveals a role in conidiation and antagonism.Molecular genetics and genomics : MGG · 2021Article
- Unconventional Secretion of Nigerolysins A fromMicroorganisms · 2020Article
- Aspergillus Species in Bronchiectasis: Challenges in the Cystic Fibrosis and Non-cystic Fibrosis Airways.Mycopathologia · 2018Review
- Proteome Profile of Aspergillus terreus Conidia at Germinating Stage: Identification of Probable Virulent Factors and Enzymes from Mycotoxin Pathways.Mycopathologia · 2017Article
- Production of a Chaetomium globosum enolase monoclonal antibody.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2014Article
- Toluene diisocyanate (TDI) disposition and co-localization of immune cells in hair follicles.Toxicological sciences : an official journal of the Society of Toxicology · 2014Article
- Fungal hemolysins.Medical mycology · 2013Review
- Development of monoclonal antibodies to recombinant terrelysin and characterization of expression in Aspergillus terreus.Journal of medical microbiology · 2012Article
- Monoclonal antibodies to hyphal exoantigens derived from the opportunistic pathogen Aspergillus terreus.Clinical and vaccine immunology : CVI · 2011Article
- RahU: an inducible and functionally pleiotropic protein in Pseudomonas aeruginosa modulates innate immunity and inflammation in host cells.Cellular immunology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Fungal hemolysins are potential virulence factors. Some fungal hemolysins belong to the aegerolysin protein family that includes cytolysins capable of lysing erythrocytes and other cells. Here, we describe a hemolysin from Aspergillus terreus called terrelysin. We used the genome sequence database to identify the terrelysin sequence based on homology with other known aegerolysins. Aspergillus terreus mRNA was isolated, transcribed to cDNA and the open reading frame for terrelysin amplified by PCR using specific primers. Using the pASK-IBA6 cloning vector, we produced recombinant terrelysin (rTerrelysin) as a fusion product in Escherichia coli. The recombinant protein was purified and using MALDI-TOF MS determined to have a mass of 16,428 Da. Circular dichroism analysis suggests the secondary structure of the protein to be predominantly β-sheet. Results from thermal denaturation of rTerrelysin show that the protein maintained the β-sheet confirmation up to 65°C. Polyclonal antibody to rTerrelysin recognized a protein of approximately 16.5 kDa in mycelial extracts from A. terreus.
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Registered trials
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