ArticleMolecular systems biology2010
A sequestration feedback determines dynamics and temperature entrainment of the KaiABC circadian clock.
Article in Molecular systems biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 66 citations in OpenAlex.
- The free-running property of circadian clocks is needed to tolerate changing photoperiods.Current biology : CB · 2026Article
- Two KaiABC systems control circadian oscillations in one cyanobacterium.Nature communications · 2024Article
- A topological mechanism for robust and efficient global oscillations in biological networks.Nature communications · 2024Article
- Determining subunit-subunit interaction from statistics of cryo-EM images: observation of nearest-neighbor coupling in a circadian clock protein complex.Nature communications · 2023Article
- Article
- Overall structure of fully assembled cyanobacterial KaiABC circadian clock complex by an integrated experimental-computational approach.Communications biology · 2022Article
- Article
- Bayesian modeling reveals metabolite-dependent ultrasensitivity in the cyanobacterial circadian clock.Molecular systems biology · 2020Article
- For Whom the Clock Ticks: Clinical Chronobiology for Infectious Diseases.Frontiers in immunology · 2020Review
- Computational modelling unravels the precise clockwork of cyanobacteria.Interface focus · 2018Review
- Molecular dynamics simulations of nucleotide release from the circadian clock protein KaiC reveal atomic-resolution functional insights.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time.Nature communications · 2018Article
- Period Robustness and Entrainability of the Kai System to Changing Nucleotide Concentrations.Biophysical journal · 2017Article
- A thermodynamically consistent model of the post-translational Kai circadian clock.PLoS computational biology · 2017Article
- Architecture and mechanism of the central gear in an ancient molecular timer.Journal of the Royal Society, Interface · 2017Review
- Discrete gene replication events drive coupling between the cell cycle and circadian clocks.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Crystal Structure of the CTP1L Endolysin Reveals How Its Activity Is Regulated by a Secondary Translation Product.The Journal of biological chemistry · 2016Article
- Toward Multiscale Models of Cyanobacterial Growth: A Modular Approach.Frontiers in bioengineering and biotechnology · 2016Review
- Mixtures of opposing phosphorylations within hexamers precisely time feedback in the cyanobacterial circadian clock.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Daily rhythms in the cyanobacterium synechococcus elongatus probed by high-resolution mass spectrometry-based proteomics reveals a small defined set of cyclic proteins.Molecular & cellular proteomics : MCP · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The circadian rhythm of the cyanobacterium Synechococcus elongatus is controlled by three proteins, KaiA, KaiB, and KaiC. In a test tube, these proteins form complexes of various stoichiometry and the average phosphorylation level of KaiC exhibits robust circadian oscillations in the presence of ATP. Using mathematical modeling, we were able to reproduce quantitatively the experimentally observed phosphorylation dynamics of the KaiABC clockwork in vitro. We thereby identified a highly non-linear feedback loop through KaiA inactivation as the key synchronization mechanism of KaiC phosphorylation. By using the novel method of native mass spectrometry, we confirm the theoretically predicted complex formation dynamics and show that inactivation of KaiA is a consequence of sequestration by KaiC hexamers and KaiBC complexes. To test further the predictive power of the mathematical model, we reproduced the observed phase synchronization dynamics on entrainment by temperature cycles. Our model gives strong evidence that the underlying entrainment mechanism arises from a temperature-dependent change in the abundance of KaiAC and KaiBC complexes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.