ArticleMolecular systems biology2010
Evolutionary fates within a microbial population highlight an essential role for protein folding during natural selection.
Article in Molecular systems biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- Rheostats, toggles, and neutrals, Oh my! A new framework for understanding how amino acid changes modulate protein function.The Journal of biological chemistry · 2024Review
- Intragenic compensation through the lens of deep mutational scanning.Biophysical reviews · 2022Review
- Advances in automated real-time flow cytometry for monitoring of bioreactor processes.Engineering in life sciences · 2022Review
- The fitness cost and benefit of phase-separated protein deposits.Molecular systems biology · 2019Article
- Using cellular fitness to map the structure and function of a major facilitator superfamily effluxer.Molecular systems biology · 2017Article
- Enzyme Efficiency but Not Thermostability Drives Cefotaxime Resistance Evolution in TEM-1 β-Lactamase.Molecular biology and evolution · 2017Article
- Using Evolution to Guide Protein Engineering: The Devil IS in the Details.Biophysical journal · 2016Review
- Leveraging the Mechanism of Oxidative Decay for Adenylate Kinase to Design Structural and Functional Resistances.ACS chemical biology · 2015Article
- Metabolism at evolutionary optimal States.Metabolites · 2015Review
- Mutations in global regulators lead to metabolic selection during adaptation to complex environments.PLoS genetics · 2014Article
- Transformable facultative thermophile Geobacillus stearothermophilus NUB3621 as a host strain for metabolic engineering.Applied microbiology and biotechnology · 2014Article
- Article
- Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to resistance.Antimicrobial agents and chemotherapy · 2013Article
- Evolutionary biochemistry: revealing the historical and physical causes of protein properties.Nature reviews. Genetics · 2013Review
- Article
- Small changes in enzyme function can lead to surprisingly large fitness effects during adaptive evolution of antibiotic resistance.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Cellular strategies for regulating functional and nonfunctional protein aggregation.Cell reports · 2012Article
- Fast stochastic algorithm for simulating evolutionary population dynamics.Bioinformatics (Oxford, England) · 2012Article
- Soluble oligomerization provides a beneficial fitness effect on destabilizing mutations.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- FREQ-Seq: a rapid, cost-effective, sequencing-based method to determine allele frequencies directly from mixed populations.PloS one · 2012Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Systems biology can offer a great deal of insight into evolution by quantitatively linking complex properties such as protein structure, folding, and function to the fitness of an organism. Although the link between diseases such as Alzheimer's and misfolding is well appreciated, directly showing the importance of protein folding to success in evolution has been more difficult. We show here that predicting success during adaptation can depend critically on enzyme kinetic and folding models. We used a 'weak link' method to favor mutations to an essential, but maladapted, adenylate kinase gene within a microbial population that resulted in the identification of five mutants that arose nearly simultaneously and competed for success. Physicochemical characterization of these mutants showed that, although steady-state enzyme activity is important, success within the population is critically dependent on resistance to denaturation and aggregation. A fitness function based on in vitro measurements of enzyme activity, reversible and irreversible unfolding, and the physiological context reproduces in vivo evolutionary fates in the population linking organismal adaptation to its physical basis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.