ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2010
Functional adaptation of the switch-2 nucleotide sensor enables rapid processive translocation by myosin-5.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 14 citations in OpenAlex.
- Switch-2 determines MgFrontiers in physiology · 2024Article
- Actomyosin Complex.Sub-cellular biochemistry · 2022Article
- Structural and Computational Insights into a Blebbistatin-Bound Myosin•ADP Complex with Characteristics of an ADP-Release Conformation along the Two-Step Myosin Power Stoke.International journal of molecular sciences · 2020Article
- Interplay of actin, ADP and MgArchives of biochemistry and biophysics · 2019Article
- Magnesium modulates actin binding and ADP release in myosin motors.The Journal of biological chemistry · 2014Article
- Magnesium impacts myosin V motor activity by altering key conformational changes in the mechanochemical cycle.Biochemistry · 2013Article
- Global fit analysis of myosin-5b motility reveals thermodynamics of Mg2+-sensitive acto-myosin-ADP states.PloS one · 2013Article
- A subdomain interaction at the base of the lever allosterically tunes the mechanochemical mechanism of myosin 5a.PloS one · 2013Article
- Switch II mutants reveal coupling between the nucleotide- and actin-binding regions in myosin V.Biophysical journal · 2012Article
- Myosin IIIB uses an actin-binding motif in its espin-1 cargo to reach the tips of actin protrusions.Current biology : CB · 2012Article
- A novel actin binding site of myosin required for effective muscle contraction.Nature structural & molecular biology · 2012Article
- Comparative kinetic and functional characterization of the motor domains of human nonmuscle myosin-2C isoforms.The Journal of biological chemistry · 2011Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Active site loops that are conserved across superfamilies of myosins, kinesins, and G proteins play key roles in allosteric coupling of NTP hydrolysis to interaction with track filaments or effector proteins. In this study, we investigated how the class-specific natural variation in the switch-2 active site loop contributes to the motor function of the intracellular transporter myosin-5. We used single-molecule, rapid kinetic and spectroscopic experiments and semiempirical quantum chemical simulations to show that the class-specific switch-2 structure including a tyrosine (Y439) in myosin-5 enables rapid processive translocation along actin filaments by facilitating Mg(2+)-dependent ADP release. Using wild-type control and Y439 point mutant myosin-5 proteins, we demonstrate that the translocation speed precisely correlates with the kinetics of nucleotide exchange. Switch-2 variants can thus be used to fine-tune translocation speed while maintaining high processivity. The class-specific variation of switch-2 in various NTPase superfamilies indicates its general role in the kinetic tuning of Mg(2+)-dependent nucleotide exchange.
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