ArticlePlant physiology2010
Identification and characterization of proteins involved in rice urea and arginine catabolism.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 58 citations in OpenAlex.
- Article
- Enzymes and cellular interplay required for flux of fixed nitrogen to ureides in bean nodules.Nature communications · 2022Article
- Transport, functions, and interaction of calcium and manganese in plant organellar compartments.Plant physiology · 2021Review
- Arginine Increases Tolerance to Nitrogen Deficiency inFrontiers in plant science · 2021Article
- A Kinase and a Glycosylase Catabolize Pseudouridine in the Peroxisome to Prevent Toxic Pseudouridine Monophosphate Accumulation.The Plant cell · 2020Article
- Manganese in Plants: From Acquisition to Subcellular Allocation.Frontiers in plant science · 2020Review
- AMP and GMP Catabolism in Arabidopsis Converge on Xanthosine, Which Is Degraded by a Nucleoside Hydrolase Heterocomplex.The Plant cell · 2019Article
- Phenotyping for Nitrogen Use Efficiency: Rice Genotypes Differ in N-Responsive Germination, Oxygen Consumption, Seed Urease Activities, Root Growth, Crop Duration, and Yield at Low N.Frontiers in plant science · 2018Article
- Coprophagous features in carnivorous Nepenthes plants: a task for ureases.Scientific reports · 2017Article
- The assembly of the plant urease activation complex and the essential role of the urease accessory protein G (UreG) in delivery of nickel to urease.The Journal of biological chemistry · 2017Article
- Comprehensive molecular analysis of arginase-encoding genes in common wheat and its progenitor species.Scientific reports · 2017Article
- Effect of soybean ureases on seed germination and plant development.Genetics and molecular biology · 2017Article
- Molecular docking of Glycine max and Medicago truncatula ureases with urea; bioinformatics approaches.Molecular biology reports · 2016Article
- Both Free Indole-3-Acetic Acid and Photosynthetic Performance are Important Players in the Response of Medicago truncatula to Urea and Ammonium Nutrition Under Axenic Conditions.Frontiers in plant science · 2016Article
- Urea retranslocation from senescing Arabidopsis leaves is promoted by DUR3-mediated urea retrieval from leaf apoplast.The Plant journal : for cell and molecular biology · 2015Article
- Uric acid accumulation in an Arabidopsis urate oxidase mutant impairs seedling establishment by blocking peroxisome maintenance.The Plant cell · 2014Article
- Plant purine nucleoside catabolism employs a guanosine deaminase required for the generation of xanthosine in Arabidopsis.The Plant cell · 2013Article
- Biochemical and structural studies on native and recombinant Glycine max UreG: a detailed characterization of a plant urease accessory protein.Plant molecular biology · 2012Article
- Mutational analysis of the major soybean UreF paralogue involved in urease activation.Journal of experimental botany · 2011Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rice (Oryza sativa) production relies strongly on nitrogen (N) fertilization with urea, but the proteins involved in rice urea metabolism have not yet been characterized. Coding sequences for rice arginase, urease, and the urease accessory proteins D (UreD), F (UreF), and G (UreG) involved in urease activation were identified and cloned. The functionality of urease and the urease accessory proteins was demonstrated by complementing corresponding Arabidopsis (Arabidopsis thaliana) mutants and by multiple transient coexpression of the rice proteins in Nicotiana benthamiana. Secondary structure models of rice (plant) UreD and UreF proteins revealed a possible functional conservation to bacterial orthologs, especially for UreF. Using amino-terminally StrepII-tagged urease accessory proteins, an interaction between rice UreD and urease could be shown. Prokaryotic and eukaryotic urease activation complexes seem conserved despite limited protein sequence conservation for UreF and UreD. In plant metabolism, urea is generated by the arginase reaction. Rice arginase was transiently expressed as a carboxyl-terminally StrepII-tagged fusion protein in N. benthamiana, purified, and biochemically characterized (K(m) = 67 mm, k(cat) = 490 s(-1)). The activity depended on the presence of manganese (K(d) = 1.3 microm). In physiological experiments, urease and arginase activities were not influenced by the external N source, but sole urea nutrition imbalanced the plant amino acid profile, leading to the accumulation of asparagine and glutamine in the roots. Our data indicate that reduced plant performance with urea as N source is not a direct result of insufficient urea metabolism but may in part be caused by an imbalance of N distribution.
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