ArticleJournal of virology2010
Characterization of a putative ancestor of coxsackievirus B5.
Article in Journal of virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- Trial
- Influence of Amino Acid Substitutions in Capsid Proteins of Coxsackievirus B5 on Free Chlorine and Thermal Inactivation.Environmental science & technology · 2024Article
- Molecular characterization of coxsackievirus B5 from the sputum of pneumonia children patients of Kunming, Southwest China.Virology journal · 2023Article
- Atomic Structures of Coxsackievirus B5 Provide Key Information on Viral Evolution and Survival.Journal of virology · 2022Article
- Genetic diversity and evolution of enterovirus A71 subgenogroup C1 from children with hand, foot, and mouth disease in Thailand.Archives of virology · 2021Article
- Immunological Study of Reconstructed Common Ancestral Sequence of Adenovirus Hexon Protein.Frontiers in microbiology · 2021Article
- Slow Infection due to Lowering the Amount of Intact versus Empty Particles Is a Characteristic Feature of Coxsackievirus B5 Dictated by the Structural Proteins.Journal of virology · 2019Article
- A neonatal mouse model of central nervous system infections caused by Coxsackievirus B5.Emerging microbes & infections · 2018Article
- Alignment Modulates Ancestral Sequence Reconstruction Accuracy.Molecular biology and evolution · 2018Article
- Evolutionary histories of coxsackievirus B5 and swine vesicular disease virus reconstructed by phylodynamic and sequence variation analyses.Scientific reports · 2018Article
- Seroepidemiology of coxsackievirus B5 in infants and children in Jiangsu province, China.Human vaccines & immunotherapeutics · 2018Article
- Analysis of an echovirus 18 outbreak in Thuringia, Germany: insights into the molecular epidemiology and evolution of several enterovirus species B members.Medical microbiology and immunology · 2016Article
- AAV ancestral reconstruction library enables selection of broadly infectious viral variants.Gene therapy · 2015Article
- Viral meningitis epidemics and a single, recent, recombinant and anthroponotic origin of swine vesicular disease virus.Evolution, medicine, and public health · 2015Article
- In Silico Reconstruction of the Viral Evolutionary Lineage Yields a Potent Gene Therapy Vector.Cell reports · 2015Article
- Efficient replication of recombinant Enterovirus B types, carrying different P1 genes in the coxsackievirus B5 replicative backbone.Virus genes · 2015Article
- The evolution of Vp1 gene in enterovirus C species sub-group that contains types CVA-21, CVA-24, EV-C95, EV-C96 and EV-C99.PloS one · 2014Article
- Phylogenetic patterns of human coxsackievirus B5 arise from population dynamics between two genogroups and reveal evolutionary factors of molecular adaptation and transmission.Journal of virology · 2013Article
- Emergence, circulation, and spatiotemporal phylogenetic analysis of coxsackievirus a6- and coxsackievirus a10-associated hand, foot, and mouth disease infections from 2008 to 2012 in Shenzhen, China.Journal of clinical microbiology · 2013Article
- In silico resurrection of the major vault protein suggests it is ancestral in modern eukaryotes.Genome biology and evolution · 2013Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 5 countries.
Funding
Abstract
Like other RNA viruses, coxsackievirus B5 (CVB5) exists as circulating heterogeneous populations of genetic variants. In this study, we present the reconstruction and characterization of a probable ancestral virion of CVB5. Phylogenetic analyses based on capsid protein-encoding regions (the VP1 gene of 41 clinical isolates and the entire P1 region of eight clinical isolates) of CVB5 revealed two major cocirculating lineages. Ancestral capsid sequences were inferred from sequences of these contemporary CVB5 isolates by using maximum likelihood methods. By using Bayesian phylodynamic analysis, the inferred VP1 ancestral sequence dated back to 1854 (1807 to 1898). In order to study the properties of the putative ancestral capsid, the entire ancestral P1 sequence was synthesized de novo and inserted into the replicative backbone of an infectious CVB5 cDNA clone. Characterization of the recombinant virus in cell culture showed that fully functional infectious virus particles were assembled and that these viruses displayed properties similar to those of modern isolates in terms of receptor preferences, plaque phenotypes, growth characteristics, and cell tropism. This is the first report describing the resurrection and characterization of a picornavirus with a putative ancestral capsid. Our approach, including a phylogenetics-based reconstruction of viral predecessors, could serve as a starting point for experimental studies of viral evolution and might also provide an alternative strategy for the development of vaccines.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.