ArticleThe Journal of biological chemistry2010
Structural and functional insights into Saccharomyces cerevisiae Tpa1, a putative prolylhydroxylase influencing translation termination and transcription.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 31 citations in OpenAlex.
- Lithium chloride sensitivity connects the activity of PEX11 and RIM20 to the translation of PGM2 and other mRNAs with structured 5'-UTRs.Molecular and cellular biochemistry · 2022Article
- Ogfod1 deletion increases cardiac beta-alanine levels and protects mice against ischaemia- reperfusion injury.Cardiovascular research · 2022Article
- Iron in Translation: From the Beginning to the End.Microorganisms · 2021Review
- Expansion of base excision repair compensates for a lack of DNA repair by oxidative dealkylation in budding yeast.The Journal of biological chemistry · 2019Article
- Oxygen-responsive transcriptional regulation of lipid homeostasis in fungi: Implications for anti-fungal drug development.Seminars in cell & developmental biology · 2018Review
- Eukaryotic translational termination efficiency is influenced by the 3' nucleotides within the ribosomal mRNA channel.Nucleic acids research · 2018Article
- Molecular and cellular mechanisms of HIF prolyl hydroxylase inhibitors in clinical trials.Chemical science · 2017Article
- Article
- Nutrient sensing and TOR signaling in yeast and mammals.The EMBO journal · 2017Review
- Nonsense Suppression as an Approach to Treat Lysosomal Storage Diseases.Diseases (Basel, Switzerland) · 2016Article
- Structure and Mechanism of a Viral Collagen Prolyl Hydroxylase.Biochemistry · 2015Article
- Structure of the ribosomal oxygenase OGFOD1 provides insights into the regio- and stereoselectivity of prolyl hydroxylases.Structure (London, England : 1993) · 2015Article
- A role for Saccharomyces cerevisiae Tpa1 protein in direct alkylation repair.The Journal of biological chemistry · 2014Article
- Fungal morphogenesis.Cold Spring Harbor perspectives in medicine · 2014Review
- Human oxygen sensing may have origins in prokaryotic elongation factor Tu prolyl-hydroxylation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- OGFOD1 catalyzes prolyl hydroxylation of RPS23 and is involved in translation control and stress granule formation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Sudestada1, a Drosophila ribosomal prolyl-hydroxylase required for mRNA translation, cell homeostasis, and organ growth.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Hydroxylation of the eukaryotic ribosomal decoding center affects translational accuracy.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Synergistic regulation of hyphal elongation by hypoxia, CO(2), and nutrient conditions controls the virulence of Candida albicans.Cell host & microbe · 2013Article
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficiency of translation termination relies on the specific recognition of the three stop codons by the eukaryotic translation termination factor eRF1. To date only a few proteins are known to be involved in translation termination in eukaryotes. Saccharomyces cerevisiae Tpa1, a largely conserved but uncharacterized protein, has been described to associate with a messenger ribonucleoprotein complex located at the 3' end of mRNAs that contains at least eRF1, eRF3, and Pab1. Deletion of the TPA1 gene results in a decrease of translation termination efficacy and an increase in mRNAs half-lives and longer mRNA poly(A) tails. In parallel, Schizosaccharomyces pombe Ofd1, a Tpa1 ortholog, and its partner Nro1 have been implicated in the regulation of the stability of a transcription factor that regulates genes essential for the cell response to hypoxia. To gain insight into Tpa1/Ofd1 function, we have solved the crystal structure of S. cerevisiae Tpa1 protein. This protein is composed of two equivalent domains with the double-stranded β-helix fold. The N-terminal domain displays a highly conserved active site with strong similarities with prolyl-4-hydroxylases. Further functional studies show that the integrity of Tpa1 active site as well as the presence of Yor051c/Ett1 (the S. cerevisiae Nro1 ortholog) are essential for correct translation termination. In parallel, we show that Tpa1 represses the expression of genes regulated by Hap1, a transcription factor involved in the response to levels of heme and oxygen. Altogether, our results support that Tpa1 is a putative enzyme acting as an oxygen sensor and influencing several distinct regulatory pathways.
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