ArticleThe Journal of membrane biology2010
Cell-cell electrofusion: optimization of electric field amplitude and hypotonic treatment for mouse melanoma (B16-F1) and Chinese Hamster ovary (CHO) cells.
Article in The Journal of membrane biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 57 citations in OpenAlex.
- Fusion of Human Synovium-Derived Mesenchymal Stem/Stromal Cells with Primary Human Chondrocytes Using the Modified Adherence Method (MAM).Methods in molecular biology (Clifton, N.J.) · 2025Article
- Feasibility Study for the Use of Gene Electrotransfer and Cell Electrofusion as a Single-Step Technique for the Generation of Activated Cancer Cell Vaccines.The Journal of membrane biology · 2024Article
- Theoretical analysis for the fluctuation in the electric parameters of the electroporated cells before and during the electrofusion.Medical & biological engineering & computing · 2022Article
- Real-time detection of somatic hybrid cells during electrofusion of carrot protoplasts with stably labelled mitochondria.Scientific reports · 2020Article
- The Effect of Lipid Antioxidant α-Tocopherol on Cell Viability and Electrofusion Yield of B16-F1 Cells In Vitro.The Journal of membrane biology · 2019Article
- Electrofusion of single cells in picoliter droplets.Scientific reports · 2018Article
- Article
- Synergistic combinations of short high-voltage pulses and long low-voltage pulses enhance irreversible electroporation efficacy.Scientific reports · 2017Article
- Cell Electrofusion in Centrifuged Erythrocyte Pellets Assessed by Dielectric Spectroscopy.The Journal of membrane biology · 2016Article
- Content Delivery of Lipidic Nanovesicles in Electropermeabilized Cells.The Journal of membrane biology · 2015Article
- Electroporation in food processing and biorefinery.The Journal of membrane biology · 2014Review
- Planning of electroporation-based treatments using Web-based treatment-planning software.The Journal of membrane biology · 2013Article
- Comparison of alkaline lysis with electroextraction and optimization of electric pulses to extract plasmid DNA from Escherichia coli.The Journal of membrane biology · 2013Article
- Dielectric analysis and multi-cell electrofusion of the yeast Pichia pastoris for electrophysiological studies.The Journal of membrane biology · 2012Article
- Network for development of electroporation-based technologies and treatments: COST TD1104.The Journal of membrane biology · 2012Article
- The systematic study of the electroporation and electrofusion of B16-F1 and CHO cells in isotonic and hypotonic buffer.The Journal of membrane biology · 2012Article
- Alteration of cellular behavior and response to PI3K pathway inhibition by culture in 3D collagen gels.PloS one · 2012Article
- A cell electrofusion microfluidic device integrated with 3D thin-film microelectrode arrays.Biomicrofluidics · 2011Article
- Use of collagen gel as a three-dimensional in vitro model to study electropermeabilization and gene electrotransfer.The Journal of membrane biology · 2010Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient electroporation of cells in physical contact induces cell fusion, and this process is known as electrofusion. It has been shown that appropriate hypotonic treatment of cells before the application of electric pulses can cause a significant increase in electrofusion efficiency. First, the amplitudes of the electric field were determined spectrofluorometrically, where sufficient permeabilization in hypotonic buffer occurred for B16-F1 and CHO cells. In further electrofusion experiments 14 +/- 4% of fused cells for B16-F1 and 6 +/- 1% for CHO was achieved. These electrofusion efficiencies, determined by double staining and fluorescence microcopy, are comparable to those of other published studies. It was also confirmed that successful electroporation does not necessarily guarantee high electrofusion efficiency due to biological factors involved in the electrofusion process. Furthermore, not only the extension of electrofusion but also cell survival depends on the cell line used. Further studies are needed to improve overall cell survival after electroporation in hypotonic buffer, which was significantly reduced, especially for B16-F1 cells. Another contribution of this report is the description of a simple modification of the adherence method for formation of spontaneous cell contact, while cells preserve their spherical shape.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.