ArticlePLoS genetics2010
A forward-genetic screen and dynamic analysis of lambda phage host-dependencies reveals an extensive interaction network and a new anti-viral strategy.
Article in PLoS genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers, 1 of them a synthesis that pooled it.
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Who cites it
57 citing papers in PubMed, 1 synthesis or guideline pooled it, 109 citations in OpenAlex.
- Statistical structure of host-phage interactions.Proceedings of the National Academy of Sciences of the United States of America · 2011Pooled it
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- Episomal virus maintenance enables bacterial population recovery from infection and promotes virus-bacterial coexistence.The ISME journal · 2025Article
- Precision targeting of genetic variations in mixed bacterial cultures using CRISPR-Cas12a-programmed λ phages.Frontiers in microbiology · 2025Article
- Inferring strain-level mutational drivers of phage-bacteria interaction phenotypes arising during coevolutionary dynamics.bioRxiv : the preprint server for biology · 2024Article
- PlzR regulates type IV pili assembly in Pseudomonas aeruginosa via PilZ binding.Nature communications · 2024Article
- Using bacterial population dynamics to count phages and their lysogens.Nature communications · 2024Article
- Soil Giant Phage: Genome and Biological Characteristics ofInternational journal of molecular sciences · 2024Article
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- Inferring strain-level mutational drivers of phage-bacteria interaction phenotypes arising during coevolutionary dynamics.Virus evolution · 2024Article
- Bacteriophage-mediated lysis supports robust growth of amino acid auxotrophs.bioRxiv : the preprint server for biology · 2023Article
- New workflow predicts drug targets against SARS-CoV-2 via metabolic changes in infected cells.PLoS computational biology · 2023Article
- Exopolysaccharide is required for motility, stress tolerance, and plant colonization by the endophytic bacteriumFrontiers in microbiology · 2023Article
- Mechanisms regulating the CRISPR-Cas systems.Frontiers in microbiology · 2023Review
- Learning from the Invaders: What Viruses Teach Us about RNA-Based Regulation in Microbes.Microorganisms · 2022Article
- Study of Ren, RexA, and RexB Functions Provides Insight Into the Complex Interaction Between Bacteriophage λ and Its Host,PHAGE (New Rochelle, N.Y.) · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Latently infecting viruses are an important class of virus that plays a key role in viral evolution and human health. Here we report a genome-scale forward-genetics screen for host-dependencies of the latently-infecting bacteriophage lambda. This screen identified 57 Escherichia coli (E. coli) genes--over half of which have not been previously associated with infection--that when knocked out inhibited lambda phage's ability to replicate. Our results demonstrate a highly integrated network between lambda and its host, in striking contrast to the results from a similar screen using the lytic-only infecting T7 virus. We then measured the growth of E. coli under normal and infected conditions, using wild-type and knockout strains deficient in one of the identified host genes, and found that genes from the same pathway often exhibited similar growth dynamics. This observation, combined with further computational and experimental analysis, led us to identify a previously unannotated gene, yneJ, as a novel regulator of lamB gene expression. A surprising result of this work was the identification of two highly conserved pathways involved in tRNA thiolation-one pathway is required for efficient lambda replication, while the other has anti-viral properties inhibiting lambda replication. Based on our data, it appears that 2-thiouridine modification of tRNAGlu, tRNAGln, and tRNALys is particularly important for the efficient production of infectious lambda phage particles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.