ArticleProceedings of the National Academy of Sciences of the United States of America1991
Delivery of macromolecules into living cells: a method that exploits folate receptor endocytosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers.
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Who cites it
112 citing papers in PubMed, 557 citations in OpenAlex.
- Folate-targeted gold nanoparticles for doxorubicin delivery in tumor spheroids.Drug delivery · 2026Article
- Folic Acid, Folate Conjugates and Folate Receptors: Novel Applications in Imaging of Cancer and Inflammation-Related Conditions.Cancer management and research · 2025Review
- Study on the diagnostic value of folic acid-targeted nano-microbubbles in breast microcarcinoma.Frontiers in oncology · 2025Article
- Laser-Induced Intracellular Delivery: Exploiting Gold-Coated Spiky Polymeric Nanoparticles and Gold Nanorods under Near-Infrared Pulses for Single-Cell Nano-Photon-Poration.Micromachines · 2024Article
- In vitro and in vivo Evaluation of Folic Acid Modified DOX-LoadedInternational journal of nanomedicine · 2023Article
- A Multifunctional Conjugated Polymer Developed as an Efficient System for Differentiation of SH-SY5Y Tumour Cells.Polymers · 2022Article
- Effect of Trimethine Cyanine Dye- and Folate-Conjugation on the In Vitro Biological Activity of Proapoptotic Peptides.Biomolecules · 2022Article
- Perspective: The High-Folate-Low-Vitamin B-12 Interaction Is a Novel Cause of Vitamin B-12 Depletion with a Specific Etiology-A Hypothesis.Advances in nutrition (Bethesda, Md.) · 2022Article
- Improved Photophysical Properties of Ionic Material-Based Combination Chemo/PDT Nanomedicine.ACS applied bio materials · 2021Article
- A Bibliometric analysis of folate receptor research.BMC cancer · 2020Article
- Targeting Tumors Using Peptides.Molecules (Basel, Switzerland) · 2020Review
- Riboflavin-Targeted Drug Delivery.Cancers · 2020Review
- Improving cellular uptake of therapeutic entities through interaction with components of cell membrane.Drug delivery · 2019Review
- Altered folate binding protein expression and folate delivery are associated with congenital hydrocephalus in the hydrocephalic Texas rat.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2019Article
- Folate Conjugated Double Liposomes Bearing Prednisolone and Methotrexate for Targeting Rheumatoid Arthritis.Pharmaceutical research · 2019Article
- Ultrabright fluorescent cellulose acetate nanoparticles for imaging tumors through systemic and topical applications.Materials today (Kidlington, England) · 2019Article
- Development of biosurfactant-based graphene quantum dot conjugate as a novel and fluorescent theranostic tool for cancer.International journal of nanomedicine · 2019Article
- Folate-targeting annonaceous acetogenins nanosuspensions: significantly enhanced antitumor efficacy in HeLa tumor-bearing mice.Drug delivery · 2018Article
- Correlating quantitative tumor accumulation and gene knockdown using SPECT/CT and bioluminescence imaging within an orthotopic ovarian cancer model.Biomaterials · 2018Article
- In Vitro and In Vivo evaluation of a novel folate-targeted theranostic nanoemulsion of docetaxel for imaging and improved anticancer activity against ovarian cancers.Cancer biology & therapy · 2018Article
52 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Difficulties with the nondestructive delivery of macromolecules into living cells have limited the potential applications of antibodies, genes, enzymes, peptides, and antisense oligonucleotides in biology and medicine. We have found, however, that the natural endocytosis pathway for the vitamin folate can be exploited to nondestructively introduce macromolecules into cultured cells if the macromolecule is first covalently linked to folate. Thus, treatment of KB cells with folate-conjugated ribonuclease, horseradish peroxidase, serum albumin, IgG, or ferritin allowed delivery of greater than 10(6) copies of the macromolecules within a 2-hr period. Cytochemical staining using 4-chloro-1-naphthol further demonstrated that the horseradish peroxidase retained activity for at least 6 hr after internalization. Since folate is an essential vitamin required in substantial quantities by virtually all cells, these observations may open the possibility of scientific and medical applications for many of the above macromolecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.