ArticleMolecular therapy : the journal of the American Society of Gene Therapy2010
Intracellular trafficking and decondensation kinetics of chitosan-pDNA polyplexes.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Stimulus-Responsive Polymeric Carriers for Gene Delivery: Balancing Endosomal Escape with Nucleic Acid Release.Pharmaceutical research · 2026Review
- Chitosan siRNA Nanoparticles Produce Significant Non-Toxic Functional Gene Silencing in Kidney Cortices.Polymers · 2024Article
- Emerging Perspectives on Prime Editor Delivery to the Brain.Pharmaceuticals (Basel, Switzerland) · 2024Review
- Antimicrobial oxygen-loaded nanobubbles as promising tools to promote wound healing in hypoxic human keratinocytes.Toxicology reports · 2022Article
- Comparative Evaluation of Different Chitosan Species and Derivatives as Candidate Biomaterials for Oxygen-Loaded Nanodroplet Formulations to Treat Chronic Wounds.Marine drugs · 2021Article
- Polyamidoamine Dendrimer Grafted with an Acid-Responsive Charge-Reversal Layer for Improved Gene Delivery.Biomacromolecules · 2020Article
- Duplex of Polyamidoamine Dendrimer/Custom-Designed Nuclear-Localization Sequence Peptide for Enhanced Gene Delivery.Bioelectricity · 2020Article
- Recent Advances in Chitosan-Based Carriers for Gene Delivery.Marine drugs · 2019Review
- Chitosan in Non-Viral Gene Delivery: Role of Structure, Characterization Methods, and Insights in Cancer and Rare Diseases Therapies.Polymers · 2018Review
- Structure Dependence of Lysosomal Transit of Chitosan-Based Polyplexes for Gene Delivery.Molecular biotechnology · 2016Article
- Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells.Scientific reports · 2015Article
- Glutamine-chitosan modified calcium phosphate nanoparticles for efficient siRNA delivery and osteogenic differentiation.Journal of materials chemistry. B · 2015Article
- Intracellular trafficking of hybrid gene delivery vectors.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- Chitin and chitosan preparation from marine sources. Structure, properties and applications.Marine drugs · 2015Review
- Real-time analysis of composite magnetic nanoparticle disassembly in vascular cells and biomimetic media.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Efficient gene delivery system mediated by cis-aconitate-modified chitosan-g-stearic acid micelles.International journal of nanomedicine · 2014Article
- Ultrasound treatment increases transfection efficiency of low molecular weight chitosan in fibroblasts but not in KB cells.PloS one · 2014Article
- Current progress in gene delivery technology based on chemical methods and nano-carriers.Theranostics · 2014Review
- Chitosans for delivery of nucleic acids.Advanced drug delivery reviews · 2013Review
- Chitosan for gene delivery and orthopedic tissue engineering applications.Molecules (Basel, Switzerland) · 2013Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The transfection efficiency (TE) of chitosan-plasmid DNA (pDNA) polyplexes can be critically modulated by the polymer degree of deacetylation (DDA) and molecular weight (MW). This study was performed to test the hypothesis that the TE dependence on chitosan MW and DDA is related to the polyplex stability, hence their intracellular decondensation/unpacking kinetics. Major barriers to nonviral gene transfer were studied by image-based quantification. Although uptake increased with increased DDA, it did not appear to be a structure-dependent process affecting TE, nor was nuclear entry. Colocalization analysis showed that all chitosans trafficked through lysosomes with similar kinetics. Fluorescent resonant energy transfer (FRET) analysis revealed a distinct relationship between TE and polyplex dissociation rate. The most efficient chitosans showed an intermediate stability and a kinetics of dissociation, which occurred in synchrony with lysosomal escape. In contrast, a rapid dissociation before lysosomal escape was found for the inefficient low DDA chitosan whereas the highly stable and inefficient complex formed by a high MW and high DDA chitosan did not dissociate even after 24 hours. This study identified that the kinetics of decondensation in relation to lysosomal escape was a most critical structure-dependent process affecting the TE of chitosan polyplexes.
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Registered trials
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