ArticleThe Journal of biological chemistry2010
CCS5, a thioredoxin-like protein involved in the assembly of plastid c-type cytochromes.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- A Metal Importer and Exporter Interact Differently in the Chloroplast and Cell Membrane.Membranes · 2026Article
- Two disulfide-reducing pathways are required for the maturation of plastid c-type cytochromes in Chlamydomonas reinhardtii.Genetics · 2023Article
- Current Knowledge on Mechanisms Preventing Photosynthesis Redox Imbalance in Plants.Antioxidants (Basel, Switzerland) · 2021Review
- Regulation of Iron Homeostasis and Use in Chloroplasts.International journal of molecular sciences · 2020Review
- Chloroplast proteome analysis of Nicotiana tabacum overexpressing TERF1 under drought stress condition.Botanical studies · 2018Article
- The DnaJ-Like Zinc-Finger Protein HCF222 Is Required for Thylakoid Membrane Biogenesis in Plants.Plant physiology · 2017Article
- Maturation of PlastidFrontiers in plant science · 2017Review
- CCS2, an Octatricopeptide-Repeat Protein, Is Required for Plastid CytochromeFrontiers in plant science · 2017Article
- Split-ubiquitin yeast two-hybrid interaction reveals a novel interaction between a natural resistance associated macrophage protein and a membrane bound thioredoxin in Brassica juncea.Plant molecular biology · 2016Article
- Nitric oxide-triggered remodeling of chloroplast bioenergetics and thylakoid proteins upon nitrogen starvation in Chlamydomonas reinhardtii.The Plant cell · 2014Article
- How to build functional thylakoid membranes: from plastid transcription to protein complex assembly.Planta · 2013Review
- Operation of trans-thylakoid thiol-metabolizing pathways in photosynthesis.Frontiers in plant science · 2013Article
- Regulatory factors for the assembly of thylakoid membrane protein complexes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- Lumen Thiol Oxidoreductase1, a disulfide bond-forming catalyst, is required for the assembly of photosystem II in Arabidopsis.The Plant cell · 2011Article
- A novel component of the disulfide-reducing pathway required for cytochrome c assembly in plastids.Genetics · 2011Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
The c-type cytochromes are metalloproteins with a heme molecule covalently linked to the sulfhydryls of a CXXCH heme-binding site. In plastids, at least six assembly factors are required for heme attachment to the apo-forms of cytochrome f and cytochrome c(6) in the thylakoid lumen. CCS5, controlling plastid cytochrome c assembly, was identified through insertional mutagenesis in the unicellular green alga Chlamydomonas reinhardtii. The complementing gene encodes a protein with similarity to Arabidopsis thaliana HCF164, which is a thylakoid membrane-anchored protein with a lumen-facing thioredoxin-like domain. HCF164 is required for cytochrome b(6)f biogenesis, but its activity and site of action in the assembly process has so far remained undeciphered. We show that CCS5 is a component of a trans-thylakoid redox pathway and operates by reducing the CXXCH heme-binding site of apocytochrome c prior to the heme ligation reaction. The proposal is based on the following findings: 1) the ccs5 mutant is rescued by exogenous thiols; 2) CCS5 interacts with apocytochrome f and c(6) in a yeast two-hybrid assay; and 3) recombinant CCS5 is able to reduce a disulfide in the CXXCH heme-binding site of apocytochrome f.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.