ArticleGenetics2010
The rapidly evolving centromere-specific histone has stringent functional requirements in Arabidopsis thaliana.
Article in Genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 114 citations in OpenAlex.
- Reproductive mode and ploidy are associated with distinct organization of clustered centromeric regions in Meloidogyne nematodes.Genome biology · 2026Article
- Targeted CENH3 protein depletion in egg cells enables highly efficient haploid induction.Plant communications · 2026Article
- Article
- Retrotransposon addiction promotes centromere function via epigenetically activated small RNAs.Nature plants · 2024Article
- The Chaperone NASP Contributes to de Novo Deposition of the Centromeric Histone Variant CENH3 in Arabidopsis Early Embryogenesis.Plant & cell physiology · 2024Article
- Centromere sequence-independent but biased loading of subgenome-specific CENH3 variants in allopolyploid Arabidopsis suecica.Plant molecular biology · 2024Article
- Native and tagged CENP-A histones are functionally inequivalent.Epigenetics & chromatin · 2024Article
- Plasticity of parental CENH3 incorporation into the centromeres in wheat × barley F1 hybrids.Frontiers in plant science · 2024Article
- Plant kinetochore complex: composition, function, and regulation.Frontiers in plant science · 2024Review
- Article
- A simple and highly efficient strategy to induce both paternal and maternal haploids through temperature manipulation.Nature plants · 2023Article
- Uncovering natural allelic and structural variants of OsCENH3 gene by targeted resequencing and in silico mining in genus Oryza.Scientific reports · 2023Article
- The Roads to Haploid Embryogenesis.Plants (Basel, Switzerland) · 2023Review
- Direct assessment of histone function using histone replacement.Trends in biochemical sciences · 2023Review
- Centromere drive: model systems and experimental progress.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2022Review
- Histone variants and modifications during abiotic stress response.Frontiers in plant science · 2022Review
- Functional Diversification of Chromatin on Rapid Evolutionary Timescales.Annual review of genetics · 2021Review
- Epigenetically mismatched parental centromeres trigger genome elimination in hybrids.Science advances · 2021Article
- The N-terminal tail ofMolecular biology of the cell · 2021Article
- Centromere Engineering as an Emerging Tool for Haploid Plant Production: Advances and Challenges.Methods in molecular biology (Clifton, N.J.) · 2021Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Centromeres control chromosome inheritance in eukaryotes, yet their DNA structure and primary sequence are hypervariable. Most animals and plants have megabases of tandem repeats at their centromeres, unlike yeast with unique centromere sequences. Centromere function requires the centromere-specific histone CENH3 (CENP-A in human), which replaces histone H3 in centromeric nucleosomes. CENH3 evolves rapidly, particularly in its N-terminal tail domain. A portion of the CENH3 histone-fold domain, the CENP-A targeting domain (CATD), has been previously shown to confer kinetochore localization and centromere function when swapped into human H3. Furthermore, CENP-A in human cells can be functionally replaced by CENH3 from distantly related organisms including Saccharomyces cerevisiae. We have used cenh3-1 (a null mutant in Arabidopsis thaliana) to replace endogenous CENH3 with GFP-tagged variants. A H3.3 tail domain-CENH3 histone-fold domain chimera rescued viability of cenh3-1, but CENH3's lacking a tail domain were nonfunctional. In contrast to human results, H3 containing the A. thaliana CATD cannot complement cenh3-1. GFP-CENH3 from the sister species A. arenosa functionally replaces A. thaliana CENH3. GFP-CENH3 from the close relative Brassica rapa was targeted to centromeres, but did not complement cenh3-1, indicating that kinetochore localization and centromere function can be uncoupled. We conclude that CENH3 function in A. thaliana, an organism with large tandem repeat centromeres, has stringent requirements for functional complementation in mitosis.
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