ArticleJournal of experimental botany2010
AHAS herbicide resistance endowing mutations: effect on AHAS functionality and plant growth.
Article in Journal of experimental botany, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 228 citations in OpenAlex.
- Pro-197 mutations confer resistance to thiencarbazone but not to foramsulfuron in ALS-resistant Poa annua populations.Pest management science · 2026Article
- Establishment of a versatile virus-based system for elucidating herbicide resistance in Galium aparine.Plant physiology · 2026Article
- AHAS structural changes caused by mutations result in differences in binding affinity and resistance to two herbicides inFrontiers in plant science · 2026Article
- Generating Broad-Spectrum Resistance to ALS-Inhibiting Herbicides in Rice by CRISPR/Cas9-Mediated NHEJ.Rice (New York, N.Y.) · 2025Article
- Widely Targeted Metabolomics Reveals Metabolic Divergence inPlants (Basel, Switzerland) · 2025Article
- Niches and Genotypes Determine the Diversity and Composition of Microbiomes After Herbicide Treatment inPlants (Basel, Switzerland) · 2025Article
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- Amino acids biosynthesis in root hair development: a mini-review.Biochemical Society transactions · 2024Review
- Distribution and Mechanism of Japanese Brome (Plants (Basel, Switzerland) · 2024Article
- The Pro-197-Thr mutation in theFrontiers in plant science · 2024Article
- Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy.Nature plants · 2023Article
- Enhanced Herbicide Metabolism and Target-Site Mutations Confer Multiple Resistance to Fomesafen and Nicosulfuron inBiology · 2023Article
- Development and validation of functional kompetitive allele-specific PCR markers for herbicide resistance inFrontiers in plant science · 2023Article
- Strategies for delivery of CRISPR/Cas-mediated genome editing to obtain edited plants directly without transgene integration.Frontiers in genome editing · 2023Review
- Review
- Growth and Competitiveness of ALS-Inhibiting Herbicide-ResistantPlants (Basel, Switzerland) · 2022Article
- Dissecting weed adaptation: Fitness and trait correlations in herbicide-resistant Alopecurus myosuroides.Pest management science · 2022Article
- Role of oxidative stress in the physiology of sensitive and resistantFrontiers in plant science · 2022Article
- Generation of Transfer-DNA-Free Base-Edited Citrus Plants.Frontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Twenty-two amino acid substitutions at seven conserved amino acid residues in the acetohydroxyacid synthase (AHAS) gene have been identified to date that confer target-site resistance to AHAS-inhibiting herbicides in biotypes of field-evolved resistant weed species. However, the effect of resistance mutations on AHAS functionality and plant growth has been investigated for only a very few mutations. This research investigates the effect of various AHAS resistance mutations in Lolium rigidum on AHAS functionality and plant growth. The enzyme kinetics of AHAS from five purified L. rigidum populations, each homozygous for the resistance mutations Pro-197-Ala, Pro-197-Arg, Pro-197-Gln, Pro-197-Ser or Trp-574-Leu, were characterized and the pleiotropic effect of three mutations on plant growth was assessed via relative growth rate analysis. All these resistance mutations endowed a herbicide-resistant AHAS and most resulted in higher extractable AHAS activity, with no-to-minor changes in AHAS kinetics. The Pro-197-Arg mutation slightly (but significantly) increased the K(m) for pyruvate and remarkably increased sensitivity to feedback inhibition by branched chain amino acids. Whereas the Pro-197-Ser and Trp-574-Leu mutations exhibited no significant effects on plant growth, the Pro-197-Arg mutation resulted in lower growth rates. It is clear that, at least in L. rigidum, these five AHAS resistance mutations have no major impact on AHAS functionality and hence probably no plant resistance costs. These results, in part, explain why so many Pro-197 AHAS resistance mutations in AHAS have evolved and why the Pro-197-Ser and the Trp-574-Leu AHAS resistance mutations are frequently found in many weed species.
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