ArticleMethods in enzymology2010
Molecules and methods for super-resolution imaging.
Article in Methods in enzymology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed.
- Targeted Photoactivatable Green-Emitting BODIPY Based on Directed Photooxidation-Induced Activation and its Application to Live Dynamic Super-Resolution Microscopy.Chemistry (Weinheim an der Bergstrasse, Germany) · 2024Article
- Small Fluorogenic Amino Acids for Peptide-Guided Background-Free Imaging.Angewandte Chemie (International ed. in English) · 2023Article
- Small Fluorogenic Amino Acids for Peptide-Guided Background-Free Imaging.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2023Article
- Obtaining 3D super-resolution images by utilizing rotationally symmetric structures and 2D-to-3D transformation.Computational and structural biotechnology journal · 2023Review
- Nanometer-Scale Molecular Mapping by Super-resolution Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Quantum Dots for Improved Single-Molecule Localization Microscopy.The journal of physical chemistry. B · 2021Article
- A biophysical perspective on receptor-mediated virus entry with a focus on HIV.Biochimica et biophysica acta. Biomembranes · 2020Review
- 3D Tracking-Free Approach for Obtaining 3D Super-Resolution Information in Rotationally Symmetric Biostructures.The journal of physical chemistry. B · 2019Article
- Switchable Fluorophores for Single-Molecule Localization Microscopy.Chemical reviews · 2018Review
- Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking.Chemical reviews · 2017Review
- Specific protein labeling with caged fluorophores for dual-color imaging and super-resolution microscopy in living cells.Chemical science · 2017Article
- Single-molecule spectroscopy and imaging over the decades.Faraday discussions · 2015Review
- Review
- Photoswitchable red fluorescent protein with a large Stokes shift.Chemistry & biology · 2014Article
- A general approach to break the concentration barrier in single-molecule imaging.Nature methods · 2012Article
- Microscopy beyond the diffraction limit using actively controlled single molecules.Journal of microscopy · 2012Review
- Image analysis in fluorescence microscopy: bacterial dynamics as a case study.BioEssays : news and reviews in molecular, cellular and developmental biology · 2012Review
- Imaging nanometer-sized α-synuclein aggregates by superresolution fluorescence localization microscopy.Biophysical journal · 2012Article
- Review of advanced imaging techniques.Journal of pathology informatics · 2012Article
- Exploring protein superstructures and dynamics in live bacterial cells using single-molecule and superresolution imaging.Methods in molecular biology (Clifton, N.J.) · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
By looking at a fluorescently labeled structure one molecule at a time, it is possible to side-step the optical diffraction limit and obtain "super-resolution" images of small nanostructures. In the Moerner Lab, we seek to develop both molecules and methods to extend super-resolution fluorescence imaging. Methodologies and protocols for designing and characterizing fluorophores with switchable fluorescence required for super-resolution imaging are reported. These fluorophores include azido-DCDHF molecules, covalently linked Cy3-Cy5 dimers, and also the first example of a photoswitchable fluorescent protein, enhanced yellow fluorescent protein (EYFP). The imaging of protein superstructures in living Caulobacter crescentus bacteria is used as an example of the power of super-resolution imaging by single-molecule photoswitching to extract information beyond the diffraction limit. Finally, a new method is described for obtaining three-dimensional super-resolution information using a double-helix point-spread function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.