ArticleBMC plant biology2010
Increasing the amylose content of durum wheat through silencing of the SBEIIa genes.
Article in BMC plant biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 182 citations in OpenAlex.
- Starch Synthase 3 isoforms are essential for normal starch granule initiation in wheat endosperm.The New phytologist · 2026Article
- Research Progress on the Regulatory Mechanism of theCurrent issues in molecular biology · 2025Review
- Food as Medicine: Curbing Type-2 Diabetes Prevalence Through Consumption of High Amylose Starchy Foods in Sub-Saharan Africa.Advanced pharmaceutical bulletin · 2025Review
- Genome-wide association analysis and transgenic characterization for amylose content regulating gene in tuber of Dioscorea zingiberensis.BMC plant biology · 2024Article
- Transcriptomics and starch biosynthesis analysis in leaves and developing seeds of mung bean provide a basis for genetic engineering of starch composition and seed quality.Frontiers in plant science · 2024Article
- Genome-wide association study identifies loci and candidate genes for RVA parameters in wheat (Frontiers in plant science · 2024Article
- Impact of Variation in Amylose Content on Durum Wheat cv. Svevo Technological and Starch Properties.Foods (Basel, Switzerland) · 2023Article
- Antisense RNA (asRNA) technology: the concept and applications in crop improvement and sustainable agriculture.Molecular biology reports · 2023Review
- Comparative Analysis of Qualitative and Bioactive Compounds of Whole and Refined Flours in Durum Wheat Grains with Different Year of Release and Yield Potential.Plants (Basel, Switzerland) · 2023Article
- Snapshot of four mature quinoa (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- BE3 is the major branching enzyme isoform required for amylopectin synthesis in CFrontiers in plant science · 2023Article
- Pregelatinized Drum-Dried Wheat Starch of Different Swelling Behavior as Clean Label Oil Replacer in Oil-in-Water Emulsions.Foods (Basel, Switzerland) · 2022Article
- Increasing the Versatility of Durum Wheat through Modifications of Protein and Starch Composition and Grain Hardness.Foods (Basel, Switzerland) · 2022Review
- Improving nutrition through biofortification-A systematic review.Frontiers in nutrition · 2022Review
- Understanding Wheat Starch Metabolism in Properties, Environmental Stress Condition, and Molecular Approaches for Value-Added Utilization.Plants (Basel, Switzerland) · 2021Review
- Starch branching enzymes as putative determinants of postharvest quality in horticultural crops.BMC plant biology · 2021Review
- Genome-wide analysis of RING-type E3 ligase family identifies potential candidates regulating high amylose starch biosynthesis in wheat (Triticum aestivum L.).Scientific reports · 2021Article
- Perspectives on the genetic improvement of health- and nutrition-related traits in pea.Plant physiology and biochemistry : PPB · 2021Review
- The Triple Jags of Dietary Fibers in Cereals: How Biotechnology IsFrontiers in plant science · 2021Review
- Non-GMO potato lines, synthesizing increased amylose and resistant starch, are mainly deficient in isoamylase debranching enzyme.Plant biotechnology journal · 2020Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundHigh amylose starch has attracted particular interest because of its correlation with the amount of Resistant Starch (RS) in food. RS plays a role similar to fibre with beneficial effects for human health, providing protection from several diseases such as colon cancer, diabetes, obesity, osteoporosis and cardiovascular diseases. Amylose content can be modified by a targeted manipulation of the starch biosynthetic pathway. In particular, the inactivation of the enzymes involved in amylopectin synthesis can lead to the increase of amylose content. In this work, genes encoding starch branching enzymes of class II (SBEIIa) were silenced using the RNA interference (RNAi) technique in two cultivars of durum wheat, using two different methods of transformation (biolistic and Agrobacterium). Expression of RNAi transcripts was targeted to the seed endosperm using a tissue-specific promoter.
resultsAmylose content was markedly increased in the durum wheat transgenic lines exhibiting SBEIIa gene silencing. Moreover the starch granules in these lines were deformed, possessing an irregular and deflated shape and being smaller than those present in the untransformed controls. Two novel granule bound proteins, identified by SDS-PAGE in SBEIIa RNAi lines, were investigated by mass spectrometry and shown to have strong homologies to the waxy proteins. RVA analysis showed new pasting properties associated with high amylose lines in comparison with untransformed controls. Finally, pleiotropic effects on other starch genes were found by semi-quantitative and Real-Time reverse transcription-polymerase chain reaction (RT-PCR).
conclusionWe have found that the silencing of SBEIIa genes in durum wheat causes obvious alterations in granule morphology and starch composition, leading to high amylose wheat. Results obtained with two different methods of transformation and in two durum wheat cultivars were comparable.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.