ArticleMolecular cancer2010
Extensive analysis of D7S486 in primary gastric cancer supports TESTIN as a candidate tumor suppressor gene.
Article in Molecular cancer, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- The value of lung function assessment and Testin expression detection in clinicopathological features and prognosis of NSCLC patients.Journal of cardiothoracic surgery · 2024Article
- Study on the expression of testin in the testes of dogs.Journal of veterinary research · 2023Article
- Diaphanous-related formin subfamily: Novel prognostic biomarkers and tumor microenvironment regulators for pancreatic adenocarcinoma.Frontiers in molecular biosciences · 2022Article
- Preliminary Study on the Expression of Testin, p16 and Ki-67 in the Cervical Intraepithelial Neoplasia.Biomedicines · 2021Article
- Silencing of Testin expression is a frequent event in spontaneous lymphomas from Trp53-mutant mice.Scientific reports · 2020Article
- The Role of Testin in Human Cancers.Pathology oncology research : POR · 2019Review
- TES functions as a Mena-dependent tumor suppressor in gastric cancer carcinogenesis and metastasis.Cancer communications (London, England) · 2019Article
- Angiogenic and Immunologic Proteins Identified by Deep Proteomic Profiling of Human Retinal and Choroidal Vascular Endothelial Cells: Potential Targets for New Biologic Drugs.American journal of ophthalmology · 2018Article
- The untold stories of the speech gene, theGenes & cancer · 2018Review
- The PET and LIM1-2 domains of testin contribute to intramolecular and homodimeric interactions.PloS one · 2017Article
- Testin (Oncology letters · 2016Article
- TESTIN Induces Rapid Death and Suppresses Proliferation in Childhood B Acute Lymphoblastic Leukaemia Cells.PloS one · 2016Article
- Downregulation of TES by hypermethylation in glioblastoma reduces cell apoptosis and predicts poor clinical outcome.European journal of medical research · 2014Article
- Promoter methylation of AREG, HOXA11, hMLH1, NDRG2, NPTX2 and Tes genes in glioblastoma.Journal of neuro-oncology · 2013Article
- The role of Rhox homeobox factors in tumorigenesis.Frontiers in bioscience (Landmark edition) · 2013Review
- Decreased expression of the FOXO3a gene is associated with poor prognosis in primary gastric adenocarcinoma patients.PloS one · 2013Article
- The Warburg effect dictates the mechanism of butyrate-mediated histone acetylation and cell proliferation.Molecular cell · 2012Article
- Testin and its emerging modulatory role in systemic carcinogenesis.Spermatogenesis · 2012Article
- Common fragile site tumor suppressor genes and corresponding mouse models of cancer.Journal of biomedicine & biotechnology · 2011Review
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHigh frequency of loss of heterozygosity (LOH) was found at D7S486 in primary gastric cancer (GC). And we found a high frequency of LOH region on 7q31 in primary GC from China, and identified D7S486 to be the most frequent LOH locus. This study was aimed to determine what genes were affected by the LOH and served as tumor suppressor genes (TSGs) in this region. Here, a high-throughput single nucleotide polymorphisms (SNPs) microarray fabricated in-house was used to analyze the LOH status around D7S486 on 7q31 in 75 patients with primary GC. Western blot, immunohistochemistry, and RT-PCR were used to assess the protein and mRNA expression of TESTIN (TES) in 50 and 140 primary GC samples, respectively. MTS assay was used to investigate the effect of TES overexpression on the proliferation of GC cell lines. Mutation and methylation analysis were performed to explore possible mechanisms of TES inactivation in GC.
resultsLOH analysis discovered five candidate genes (ST7, FOXP2, MDFIC, TES and CAV1) whose frequencies of LOH were higher than 30%. However, only TES showed the potential to be a TSG associated with GC. Among 140 pairs of GC samples, decreased TES mRNA level was found in 96 (68.6%) tumor tissues when compared with matched non-tumor tissues (p < 0.001). Also, reduced TES protein level was detected in 36 (72.0%) of all 50 tumor tissues by Western blot (p = 0.001). In addition, immunohistochemical staining result was in agreement with that of RT-PCR and Western blot. Down regulation of TES was shown to be correlated with tumor differentiation (p = 0.035) and prognosis (p = 0.035, log-rank test). Its overexpression inhibited the growth of three GC cell lines. Hypermethylation of TES promoter was a frequent event in primary GC and GC cell lines. However, no specific gene mutation was observed in the coding region of the TES gene.
conclusionsCollectively, all results support the role of TES as a TSG in gastric carcinogenesis and that TES is inactivated primarily by LOH and CpG island methylation.
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