ArticleTraffic (Copenhagen, Denmark)2010
Quantitative analysis of endocytosis with cytoplasmic pHluorin chimeras.
Article in Traffic (Copenhagen, Denmark), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed.
- ExoFILT: transfer learning for robust and accelerated analysis of exocytosis single-particle tracking data.Bioinformatics (Oxford, England) · 2026Article
- The exocyst is an insulin-sensitive regulator of amyloid precursor protein trafficking and amyloid-beta generation in neurons.bioRxiv : the preprint server for biology · 2026Article
- Sphingolipid regulation by yeast Mdm1 supports adaptive remodeling of the methionine transporter Mup1.bioRxiv : the preprint server for biology · 2026Article
- Recovery of plasma membrane tension after a hyperosmotic shock.Molecular biology of the cell · 2025Article
- Optimization of the fluorogen-activating protein tag for quantitative protein trafficking and colocalization studies inMolecular biology of the cell · 2024Article
- A novel fluorescence-activated cell sorting (FACS)-based screening identified ATG14, the gene required for pexophagy in the methylotrophic yeast.FEMS yeast research · 2024Article
- Actin- and microtubule-based motors contribute to clathrin-independent endocytosis in yeast.Molecular biology of the cell · 2023Article
- Art2 mediates selective endocytosis of methionine transporters during adaptation to sphingolipid depletion.Journal of cell science · 2023Article
- Use of Deubiquitinase Fusion Proteins to Characterize Endocytic Trafficking in Yeast.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A two-tiered system for selective receptor and transporter protein degradation.PLoS genetics · 2022Article
- Article
- Article
- Article
- ESCRT-dependent protein sorting is required for the viability of yeast clathrin-mediated endocytosis mutants.Traffic (Copenhagen, Denmark) · 2020Article
- A Snf1-related nutrient-responsive kinase antagonizes endocytosis in yeast.PLoS genetics · 2020Article
- Functional complementation reveals that 9 of the 13 human V-ATPase subunits can functionally substitute for their yeast orthologs.The Journal of biological chemistry · 2019Article
- Article
- Methionine triggers Ppz-mediated dephosphorylation of Art1 to promote cargo-specific endocytosis.The Journal of cell biology · 2019Article
- The intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation.Nature communications · 2018Article
- Eisosomes are metabolically regulated storage compartments for APC-type nutrient transporters.Molecular biology of the cell · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The pH-sensitive green fluorescent protein (GFP) variant pHluorin is typically fused to the extracellular domain of transmembrane proteins to monitor endocytosis. Here, we have turned pHluorin inside-out, and show that cytoplasmic fusions of pHluorin are effective quantitative reporters for endocytosis and multivesicular body (MVB) sorting. In yeast in particular, fusion of GFP and its variants on the extracellular side of transmembrane proteins can result in perturbed trafficking. In contrast, cytoplasmic fusions are well tolerated, allowing for the quantitative assessment of trafficking of virtually any transmembrane protein. Quenching of degradation-resistant pHluorin in the acidic vacuole permits quantification of extravacuolar cargo proteins at steady-state levels and is compatible with kinetic analysis of endocytosis in live cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.