ArticleNucleic acids research1991
Oligonucleotide inhibition of IL2R alpha mRNA transcription by promoter region collinear triplex formation in lymphocytes.
Article in Nucleic acids research, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 180 citations in OpenAlex.
- Sequence-specific triple helix formation with genomic DNA.Biochemistry · 2007Article
- Bioconjugation of oligonucleotides for treating liver fibrosis.Oligonucleotides · 2007Review
- Therapeutic modulation of endogenous gene function by agents with designed DNA-sequence specificities.Nucleic acids research · 2003Review
- Proton exchange and local stability in a DNA triple helix containing a G.TA triad.Nucleic acids research · 2001Article
- Blocking transcription of the human rhodopsin gene by triplex-mediated DNA photocrosslinking.Nucleic acids research · 2000Article
- Unambiguous demonstration of triple-helix-directed gene modification.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Poly(L-lysine)-graft-dextran copolymer: amazing effects on triplex stabilization under physiological pH and ionic conditions (in vitro).Nucleic acids research · 1998Article
- Extension of the range of DNA sequences available for triple helix formation: stabilization of mismatched triplexes by acridine-containing oligonucleotides.Nucleic acids research · 1997Article
- Targeting of the HIV-1 long terminal repeat with chromomycin potentiates the inhibitory effects of a triplex-forming oligonucleotide on Sp1-DNA interactions and in vitro transcription.The Biochemical journal · 1997Article
- Antiparallel polypurine phosphorothioate oligonucleotides form stable triplexes with the rat alpha1(I) collagen gene promoter and inhibit transcription in cultured rat fibroblasts.Nucleic acids research · 1997Article
- Investigation of the formation and intracellular stability of purine.(purine/pyrimidine) triplexes.Nucleic acids research · 1997Article
- Potassium-resistant triple helix formation and improved intracellular gene targeting by oligodeoxyribonucleotides containing 7-deazaxanthine.Nucleic acids research · 1997Article
- Accessibility of nuclear DNA to triplex-forming oligonucleotides: the integrated HIV-1 provirus as a target.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- In vivo persistence of DNA triple helices containing psoralen-conjugated oligodeoxyribonucleotides.Nucleic acids research · 1996Article
- Detection of covalent triplex within human cells.Nucleic acids research · 1996Article
- Structural specificity effects of trivalent polyamine analogues on the stabilization and conformational plasticity of triplex DNA.The Biochemical journal · 1996Article
- Triplex DNA in the nucleus: direct binding of triplex-specific antibodies and their effect on transcription, replication and cell growth.The Biochemical journal · 1996Article
- Binding of DNA oligonucleotides to sequences in the promoter of the human bc1-2 gene.Nucleic acids research · 1996Article
- Antisense oncogene and tumor suppressor gene therapy of cancer.Journal of molecular medicine (Berlin, Germany) · 1996Review
- Effect of a triplex-binding ligand on triple helix formation at a site within a natural DNA fragment.The Biochemical journal · 1996Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The promoter region of the IL2R alpha gene 5' flanking sequence contains enhancer elements crucial for binding nuclear factors which upregulate transcription following T lymphocyte activation. A 3' exonuclease resistant oligonucleotide (3'A-IL28p, terminated by a free amine group at its 3' end) was designed to bind to the IL2R alpha promoter region from -273 to -246, forming a collinear triplex spanning the kappa B enhancer (-266 to -256) as well as most of the serum response element (CArG box, -251 to -244). The binding site specificity of this oligonucleotide was demonstrated in electrophoretic mobility shift assays and by inhibition of restriction endonuclease (HinfI) cleavage within the segment of the target DNA predicted to form a triplex with the oligonucleotide. Intact normal lymphocytes, preincubated for 2h with 3'A-IL28p, accumulated less IL2Ralpha mRNA relative to other mRNAs (c-myc, beta-actin, IL2R beta, IL-6) for up to 12h after PHA stimulation, than did lymphocytes treated with a control oligomer of similar composition but different sequence. Nuclear run-on studies demonstrated that the rate of IL2R alpha mRNA synthesis relative to c-myc and beta-actin was also selectively diminished by treatment with 3'A-IL28p. These experiments suggest that transcription of individual genes can be selectively modulated in living cells by sequence specific collinear triplex formation in regulatory enhancer sequences.
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