ArticleNucleic acids research1991
Detection of an unusual distortion in A-tract DNA using KMnO4: effect of temperature and distamycin on the altered conformation.
Article in Nucleic acids research, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 32 citations in OpenAlex.
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- Thymine dimer-induced structural changes to the DNA duplex examined with reactive probes (†).Biochemistry · 2008Article
- Purine analog substitution of the HIV-1 polypurine tract primer defines regions controlling initiation of plus-strand DNA synthesis.Nucleic acids research · 2007Article
- Structure of the MutL C-terminal domain: a model of intact MutL and its roles in mismatch repair.The EMBO journal · 2004Article
- DNA interactions of antitumor cisplatin analogs containing enantiomeric amine ligands.Biophysical journal · 2000Article
- Biochemical and genetic characterization of the dominant positive element driving transcription ofthe yeast TBP-encoding gene, SPT15.Nucleic acids research · 1998Article
- Thermodynamic effects of formamide on DNA stability.Nucleic acids research · 1996Article
- The trinucleotide repeat sequence d(GTC)15 adopts a hairpin conformation.Nucleic acids research · 1995Article
- Hairpin properties of single-stranded DNA containing a GC-rich triplet repeat: (CTG)15.Nucleic acids research · 1995Article
- Structural characterization of intrinsically curved AT-rich DNA sequences.Nucleic acids research · 1994Article
- A structural analysis of the bent kinetoplast DNA from Crithidia fasciculata by high resolution chemical probing.Nucleic acids research · 1993Article
- HPLC photofingerprinting of conformational peculiarities and transitions in oligonucleotide duplexes.Nucleic acids research · 1993Article
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2 authors at 1 institution in 1 country.
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Abstract
The chemical probes potassium permanganate (KMnO4) and diethylpyrocarbonate (DEPC) can be used to study the conformational flexibility of short tracts of adenine (A-tracts) present in DNA. With these probes, we demonstrate that a novel distortion is induced in a 5 base pair A-tract at low temperature. Formation of this distorted A-tract structure, which occurs in a DNA fragment from the promoter region of the plasmid pBR322, is distinguished by a dramatic increase in the KMnO4 reactivity of the central thymines in this tract at 12 degrees C. This alteration occurs in the absence of any detectable rearrangement in the conformation of the adenines in the complementary strand. Induction of this low temperature A-tract structure is blocked by the minor groove binding drug distamycin. Hydroxyl radical footprinting of distamycin binding to the fragment containing the d(A)5 tract at 12 degrees C suggests that this drug has two different modes of binding to DNA in agreement with recent NMR data. These experiments show that short A-tracts are capable of forming more than one structural variant of B DNA in solution. The possible relationship between the intrinsic bending of DNA containing short phased A-tracts and the low temperature A-tract conformation is discussed.
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