ArticleCurrent microbiology2011
Cholesterol dependence of pseudorabies herpesvirus entry.
Article in Current microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Viral fusion proteins of classes II and III recognize and reorganize complex biological membranes.Communications biology · 2025Article
- Pseudorabies virus uses clathrin mediated endocytosis to enter PK15 swine cell line.Frontiers in microbiology · 2024Article
- Genomic regions associated with pseudorabies virus infection status in naturally infected feral swine (Frontiers in genetics · 2023Article
- Cholesterol of lipid rafts is a key determinant for entry and post-entry control of porcine rotavirus infection.BMC veterinary research · 2018Article
- Structure-Function Dissection of Pseudorabies Virus Glycoprotein B Fusion Loops.Journal of virology · 2018Article
- Cholesterol-rich lipid rafts play an important role in the Cyprinid herpesvirus 3 replication cycle.Veterinary microbiology · 2015Article
- Phage display for identifying peptides that bind the spike protein of transmissible gastroenteritis virus and possess diagnostic potential.Virus genes · 2015Article
- A phage-displayed peptide recognizing porcine aminopeptidase N is a potent small molecule inhibitor of PEDV entry.Virology · 2014Article
- Transmissible gastroenteritis virus: identification of M protein-binding peptide ligands with antiviral and diagnostic potential.Antiviral research · 2013Article
- Reverse transcription loop-mediated isothermal amplification for rapid detection of transmissible gastroenteritis virus.Current microbiology · 2011Article
- Entry pathways of herpes simplex virus type 1 into human keratinocytes are dynamin- and cholesterol-dependent.PloS one · 2011Article
- Cholesterol is important for a post-adsorption step in the entry process of transmissible gastroenteritis virus.Antiviral research · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipid rafts are special microdomains in the plasma membrane. They are enriched in sphingolipids and cholesterol, playing critical roles in many biological processes. The purpose of this study is to analyze the requirement of cholesterol, a crucial component of lipid rafts for cell infection by pseudorabies virus (PrV). Cholesterol of plasma membrane or viral envelope was depleted with methyl-beta-cyclodextrin (MβCD), and the infectivity of three strains of PrV was determined with plaque assays. The effect of adding cholesterol to MβCD-treated cells and viruses on cell infection was analyzed. Furthermore, effect of post-adsorption cholesterol depletion on PrV infection was investigated. We show that cholesterol depletion of either the plasma membrane or the viral membrane by MβCD significantly impaired the infectivity of PrV strains Kaplan, Becker, and Bartha K-61. The virus was shown to have lower cholesterol content and to respond to lower MβCD concentrations. Exogenous cholesterol added to either MβCD-treated cells or virions partially restored the virus infectivity. Optimal PrV infection requires cholesterol in viral and plasma membranes.
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