ArticleProceedings of the National Academy of Sciences of the United States of America2010
Surprising gene expression patterns within and between PDF-containing circadian neurons in Drosophila.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
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Who cites it
106 citing papers in PubMed, 170 citations in OpenAlex.
- A Tale of Two States: Similarities and Differences in Neuropeptide Signaling in Larval and Adult Drosophila.The Journal of comparative neurology · 2026Review
- Anticipatory capture of circulating peptidergic vesicles in a clock neuron.Molecular biology of the cell · 2026Article
- Octopamine receptors inFrontiers in molecular neuroscience · 2026Article
- A one-day journey to the suburbs: circadian clock in the Drosophila visual system.The FEBS journal · 2025Review
- The ubiquitin-proteasome system in circadian regulation.Frontiers in neuroscience · 2025Review
- Reassessing the involvement of the CREB pathway in the circadian clock of Drosophila melanogaster.Npj biological timing and sleep · 2024Article
- The Drosophila circadian clock gene cycle controls the development of clock neurons.PLoS genetics · 2024Article
- Circadian Rhythm Regulation by Pacemaker Neuron Chloride Oscillation in Flies.Physiology (Bethesda, Md.) · 2024Review
- Autophagy as a new player in the regulation of clock neurons physiology of Drosophila melanogaster.Scientific reports · 2024Article
- Article
- Restoration of Sleep and Circadian Behavior by Autophagy Modulation in Huntington's Disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Stable Expression of dmiR-283 in the Brain Promises Positive Effects in Endurance Exercise on Sleep-Wake Behavior in AgingInternational journal of molecular sciences · 2023Article
- Hsp40 overexpression in pacemaker neurons delays circadian dysfunction in a Drosophila model of Huntington's disease.Disease models & mechanisms · 2022Article
- Regulation of PDF receptor signaling controlling daily locomotor rhythms in Drosophila.PLoS genetics · 2022Article
- The microtubule-associated protein Tau suppresses the axonal distribution of PDF neuropeptide and mitochondria in circadian clock neurons.Human molecular genetics · 2022Article
- Chloride oscillation in pacemaker neurons regulates circadian rhythms through a chloride-sensing WNK kinase signaling cascade.Current biology : CB · 2022Article
- Systematic modeling-driven experiments identify distinct molecular clockworks underlying hierarchically organized pacemaker neurons.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Glia-Neurons Cross-Talk Regulated Through Autophagy.Frontiers in physiology · 2022Article
- Light exposure during development affects physiology of adults inFrontiers in physiology · 2022Article
- The E3 ubiquitin ligase adaptorProceedings of the National Academy of Sciences of the United States of America · 2021Article
46 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
To compare circadian gene expression within highly discrete neuronal populations, we separately purified and characterized two adjacent but distinct groups of Drosophila adult circadian neurons: the 8 small and 10 large PDF-expressing ventral lateral neurons (s-LNvs and l-LNvs, respectively). The s-LNvs are the principal circadian pacemaker cells, whereas recent evidence indicates that the l-LNvs are involved in sleep and light-mediated arousal. Although half of the l-LNv-enriched mRNA population, including core clock mRNAs, is shared between the l-LNvs and s-LNvs, the other half is l-LNv- and s-LNv-specific. The distribution of four specific mRNAs is consistent with prior characterization of the four encoded proteins, and therefore indicates successful purification of the two neuronal types. Moreover, an octopamine receptor mRNA is selectively enriched in l-LNvs, and only these neurons respond to in vitro application of octopamine. Dissection and purification of l-LNvs from flies collected at different times indicate that these neurons contain cycling clock mRNAs with higher circadian amplitudes as well as at least a 10-fold higher fraction of oscillating mRNAs than all previous analyses of head RNA. Many of these cycling l-LNv mRNAs are well expressed but do not cycle or cycle much less well elsewhere in heads. The results suggest that RNA cycling is much more prominent in circadian neurons than elsewhere in heads and may be particularly important for the functioning of these neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.