ArticleThe Journal of biological chemistry2010
Analysis of mutually exclusive alternatively spliced serpin-1 isoforms and identification of serpin-1 proteinase complexes in Manduca sexta hemolymph.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Serpin-1a and serpin-6 regulate the Toll pathway immune homeostasis by synergistically inhibiting the Spätzle-processing enzyme CLIP2 in silkworm, Bombyx mori.PLoS pathogens · 2023Article
- The Manduca sexta serpinome: Analysis of serpin genes and proteins in the tobacco hornworm.Insect biochemistry and molecular biology · 2018Article
- Manduca sexta serpin-12 controls the prophenoloxidase activation system in larval hemolymph.Insect biochemistry and molecular biology · 2018Article
- Serpin-9 and -13 regulate hemolymph proteases during immune responses of Manduca sexta.Insect biochemistry and molecular biology · 2017Article
- Manduca sexta hemolymph protease-1, activated by an unconventional non-proteolytic mechanism, mediates immune responses.Insect biochemistry and molecular biology · 2017Article
- Serpins in arthropod biology.Seminars in cell & developmental biology · 2017Review
- A Venom Serpin Splicing Isoform of the Endoparasitoid Wasp Pteromalus puparum Suppresses Host Prophenoloxidase Cascade by Forming Complexes with Host Hemolymph Proteinases.The Journal of biological chemistry · 2017Article
- Changes in the Plasma Proteome of Manduca sexta Larvae in Relation to the Transcriptome Variations after an Immune Challenge: Evidence for High Molecular Weight Immune Complex Formation.Molecular & cellular proteomics : MCP · 2016Article
- Translational Control of Host Gene Expression by a Cys-Motif Protein Encoded in a Bracovirus.PloS one · 2016Article
- Molecular Characterization and Bioinformatics Analysis of a Prophenoloxidase-1 (PPO1) in Plutella xylostella.International journal of insect science · 2016Article
- Rhipicephalus microplus serine protease inhibitor family: annotation, expression and functional characterisation assessment.Parasites & vectors · 2015Article
- Manduca sexta proprophenoloxidase activating proteinase-3 (PAP3) stimulates melanization by activating proPAP3, proSPHs, and proPOs.Insect biochemistry and molecular biology · 2014Article
- RNAi-mediated knockdown of serine protease inhibitor genes increases the mortality of Plutella xylostella challenged by destruxin A.PloS one · 2014Article
- Manduca sexta serpin-7, a putative regulator of hemolymph prophenoloxidase activation.Insect biochemistry and molecular biology · 2013Article
- Identification of plasma proteinase complexes with serpin-3 in Manduca sexta.Insect biochemistry and molecular biology · 2012Article
- Serpin-1 splicing isoform J inhibits the proSpätzle-activating proteinase HP8 to regulate expression of antimicrobial hemolymph proteins in Manduca sexta.Developmental and comparative immunology · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mutually exclusive alternative splicing produces transcripts for 12 serpin-1 isoforms in Manduca sexta that differ only in the region encoding the carboxyl-terminal 36-40-amino acid residues. This variable region includes the reactive center loop, which determines the inhibitory selectivity of the serpin. We investigated mRNA levels of individual serpin-1 isoforms by quantitative PCR. The 12 isoforms were expressed at similar levels in hemocytes, but in fat body isoform B mRNA was present at significantly higher levels than isoforms C, D, E, F, G, J, K, and Z. To investigate the presence of individual serpin-1 isoforms in plasma we used immunoaffinity purification of serpin-1 isoforms from M. sexta plasma, followed by two-dimensional PAGE and identification of protein spots by digestion with a series of proteinases and analysis of the resulting peptides by MALDI-TOF/TOF. We identified nine of the 12 serpin-1 isoforms and, through analysis of putative serpin-1-proteinase complexes, identified three endogenous M. sexta proteinase targets of serpin-1. Our results suggest that M. sexta serpin-1 isoforms A, E, and J can inhibit hemolymph proteinase 8, which activates the cytokine spätzle. At least one isoform of serpin-1 can inhibit hemocyte proteinase 1, another M. sexta blood proteinase. In addition, a complex of serpin-1K in a complex with M. sexta midgut chymotrypsin was identified, suggesting serpin-1 isoforms may also function to protect insect tissues from digestive proteinases that may leak into the hemocoel.
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