ArticleGenes & development2010
Polycistronic pre-mRNA processing in vitro: snRNP and pre-mRNA role reversal in trans-splicing.
Article in Genes & development, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Evolution of SL-RNA Genes and Their Splicing Targets in Parasitic Flatworms.Molecular biology and evolution · 2025Article
- SLRanger: an integrated approach for spliced leader detection and operon prediction using long RNA reads.Briefings in bioinformatics · 2025Article
- A nematode-specific ribonucleoprotein complex mediates interactions between the major nematode spliced leader snRNP and its target pre-mRNAs.Nucleic acids research · 2024Article
- Characterization ofNAR genomics and bioinformatics · 2024Article
- The ubiquitin-like protein Hub1/UBL-5 functions in pre-mRNA splicing in Caenorhabditis elegans.FEBS letters · 2023Article
- Resolution of polycistronic RNA by SL2RNA (New York, N.Y.) · 2020Article
- mRNA Editing, Processing and Quality Control inGenetics · 2020Review
- Trans-splicing and operons in C. elegans.WormBook : the online review of C. elegans biology · 2012Review
- Identification of transcription start sites of trans-spliced genes: uncovering unusual operon arrangements.RNA (New York, N.Y.) · 2011Article
- SL trans-splicing in a Caenorhabditis elegans in vitro extract.Cold Spring Harbor protocols · 2011Article
- A global analysis of C. elegans trans-splicing.Genome research · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Spliced leader (SL) trans-splicing in Caenorhabditis elegans attaches a 22-nucleotide (nt) exon onto the 5' end of many mRNAs. A particular class of SL, SL2, splices mRNAs of downstream operon genes. Here we use an embryonic extract-based in vitro splicing system to show that SL2 specificity information is encoded within the polycistronic pre-mRNA, and that trans-splicing specificity is recapitulated in vitro. We define an RNA sequence required for SL2 trans-splicing, the U-rich (Ur) element, through mutational analysis and bioinformatics as a short stem-loop followed by a sequence motif, UAYYUU, located approximately 50 nt upstream of the trans-splice site. Furthermore, this element is predicted in intercistronic regions of numerous operons of C. elegans and other species that use SL2 trans-splicing. We propose that the UAYYUU motif hybridizes with the 5' splice site on the SL2 RNA to recruit the SL to the pre-mRNA. In this way, the UAYYUU motif in the pre-mRNA would serve an analogous function to the similar sequence in the U1 snRNA, which binds to the 5' splice site of introns, effectively reversing the roles of snRNP and pre-mRNA in trans-splicing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.