ArticleNucleic acids research2010
Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance biosensor study.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 74 citations in OpenAlex.
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- Calcium-Dependent Chemiluminescence Catalyzed by a Truncated c-MYC Promoter G-Triplex DNA.Molecules (Basel, Switzerland) · 2024Article
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- Molecular Recognition in Confined Space Elucidated with DNA Nanopores and Single-Molecule Force Microscopy.Nano letters · 2023Article
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- Highly multiplexed tissue imaging using repeated oligonucleotide exchange reaction.European journal of immunology · 2021Article
- DNA hybridisation kinetics using single-molecule fluorescence imaging.Essays in biochemistry · 2021Review
- Divalent Cation Dependence Enhances Dopamine Aptamer Biosensing.ACS applied materials & interfaces · 2021Article
- Ensemble Sensing Using Single-Molecule DNA Copolymers.Analytical chemistry · 2020Article
- Tuning of Oxidation Potential of Ferrocene for Ratiometric Redox Labeling and Coding of Nucleotides and DNA.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Duplex Electrochemical DNA Sensor to DetectACS omega · 2019Article
- An Electrochemical DNA Biosensor for Carcinogenicity of Anticancer Compounds Based on Competition between Methylene Blue and Oligonucleotides.Sensors (Basel, Switzerland) · 2019Article
- Sequence-specific DNA solid-phase extraction in an on-chip monolith: Towards detection of antibiotic resistance genes.Journal of chromatography. A · 2017Article
- The human mitochondrial single-stranded DNA-binding protein displays distinct kinetics and thermodynamics of DNA binding and exchange.The Journal of biological chemistry · 2017Article
- A RNA nanotechnology platform for a simultaneous two-in-one siRNA delivery and its application in synergistic RNAi therapy.Scientific reports · 2016Article
- Molecularly resolved label-free sensing of single nucleobase mismatches by interfacial LNA probes.Nucleic acids research · 2016Article
- Design of a platform technology for systemic delivery of siRNA to tumours using rolling circle transcription.Nature communications · 2015Article
- Structured oligonucleotides for target indexing to allow single-vessel PCR amplification and solid support microarray hybridization.The Analyst · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Solid-phase hybridization, i.e. the process of recognition between DNA probes immobilized on a solid surface and complementary targets in a solution is a central process in DNA microarray and biosensor technologies. In this work, we investigate the simultaneous effect of monovalent and divalent cations on the hybridization of fully complementary or partly mismatched DNA targets to DNA probes immobilized on the surface of a surface plasmon resonance sensor. Our results demonstrate that the hybridization process is substantially influenced by the cation shielding effect and that this effect differs substantially for solid-phase hybridization, due to the high surface density of negatively charged probes, and hybridization in a solution. In our study divalent magnesium is found to be much more efficient in duplex stabilization than monovalent sodium (15 mM Mg2+ in buffer led to significantly higher hybridization than even 1 M Na+). This trend is opposite to that established for oligonucleotides in a solution. It is also shown that solid-phase duplex destabilization substantially increases with the length of the involved oligonucleotides. Moreover, it is demonstrated that the use of a buffer with the appropriate cation composition can improve the discrimination of complementary and point mismatched DNA targets.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.